A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli.

Acrylic acid is a value-added chemical used in industry to produce diapers, coatings, paints, and adhesives, among many others. Due to its economic importance, there is currently a need for new and sustainable ways to synthesise it. Recently, the focus has been laid in the use of Escherichia coli to express the full bio-based pathway using 3-hydroxypropionate as an intermediary through three distinct pathways (glycerol, malonyl-CoA, and β-alanine).

Reconstructing High-Quality Large-Scale Metabolic Models with merlin.

Here, the basic principles of reconstructing genome-scale metabolic models with merlin are described. This tool covers the basic stages of this process, providing several tools that allow assembling models, using the sequenced genome as a starting point. merlin has two main modules, separating the process of annotating (enzymes, transporters, and compartments) on the genome from the process of model assembly, though information from the former is integrated in the latter after curation.