Getting the proto-Pax by the tail.

Pax genes encode transcription factors governing the determination of different cell types and even organs in the development of multicellular animals. Pax proteins are characterized by the presence of three evolutionarily conserved elements: two DNA-binding domains, the paired domain (PD) and paired-type homeodomain (PtHD), and the short octopeptide sequence (OP) located between PD and PtHD. PD is the defining feature of this class of genes, while OP and/or PtHD may be divergent or absent in some members of the family.

An anthropoid-specific segmental duplication on human chromosome 1q22.

Segmental duplications (SDs) play a key role in genome evolution by providing material for gene diversification and creation of variant or novel functions. They also mediate recombinations, resulting in microdeletions, which have occasionally been associated with human genetic diseases. Here, we present a detailed analysis of a large genomic region (about 240 kb), located on human chromosome 1q22, that contains a tandem SD, SD1q22.

Comparative expression analysis of Pax3 and Pax7 during mouse myogenesis.

Pax3 and Pax7 are closely related transcription factors involved in the commitment of myogenic precursors in the developing trunk. However, it is not yet clear whether these genes are required for myogenic cell specification in the head and for post-somitic myogensis per se. In part, this uncertainty is due to the scarce information about their normal time course and pattern of expression.

Expression of two protein isoforms of PAX7 is controlled by competing cleavage-polyadenylation and splicing.

The PAX7 gene encodes an evolutionary conserved transcription factor that is involved in the determination of the myogenic cell lineage during the development of vertebrates. In the postnatal period, the function of PAX7 is ultimately required for the specification of muscle satellite cells. The fact that PAX7 is expressed in fast proliferating embryonal myoblasts and in quiescent satellite cells of adults raised the question whether different PAX7 protein isoforms may have distinct roles in these myogenic precursors.