The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15-4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology.

Capsular polysaccharide (CPS) is a heteroglycan that coats the cell surface of most isolates of the important Gram-negative bacterial pathogen, Acinetobacter baumannii. Strain MAR 15-4076, a clinical isolate recovered in Russia in 2015, was found to carry the KL129 sequence at the CPS biosynthesis K locus. The CPS was isolated from the strain and studied by sugar analysis, Smith degradation, one- and two-dimensional H and C NMR spectroscopy.

Non- Species: Occurrence in Infections in Hospitalized Patients, Identification, and Antibiotic Resistance.

Background: species other than are becoming increasingly more important as opportunistic pathogens for humans. The primary aim of this study was to assess the prevalence, species distribution, antimicrobial resistance patterns, and carbapenemase gene content of clinical non- () isolates that were collected as part of a sentinel surveillance program of bacterial infections in hospitalized patients. The secondary aim was to evaluate the performance of MALDI-TOF MS systems for the species-level identification of isolates.

Detection of carbapenemase-producing Enterobacterales by means of matrix-assisted laser desorption ionization time-of-flight mass spectrometry with ertapenem susceptibility-testing disks as source of carbapenem substrate.

MALDI-TOF mass spectrometry has become widely used in clinical microbiology and has proved highly accurate for detection of carbapenemases in Gram-negative bacteria. However, the use of carbapenem-hydrolysis assays in routine diagnostics is hampered by the need for antibiotic substances and for making their fresh solutions each time an assay is conducted. Here, we evaluated the use of commercial antibiotic susceptibility-testing disks as source of ertapenem substrate in MALDI-TOF MS-based assay for detection of carbapenemase-producing Enterobacterales (CPE).

Molecular Epidemiology of -Positive and Isolates: Results from Russian Sentinel Surveillance (2013-2018).

Background: The dissemination of mobile colistin resistance () genes is a serious healthcare threat because polymyxins represent "last-line" therapeutics for multi-drug-resistant Gram-negative pathogens. This study aimed to assess the prevalence of colistin resistance and genes and characteristics of clinical () and () isolates and plasmids carrying these genes in Russia.

Methods: A total of 4324 and 4530 collected in the frame of sentinel surveillance in 2013-2018 were tested for susceptibility to colistin and other antibiotics using the broth microdilution method.

Antibiotic Resistance and Genotypes of Nosocomial Strains of in Kazakhstan.

The aim of this study was to determine the prevalence of antibiotic-resistant strains in Kazakhstan and to characterize genotypes related to epidemic "high-risk" clones. Two hundred and twenty four isolates from four cities of Kazakhstan in 2011-2019 were studied. Antibiotic susceptibility testing was performed by using broth microdilutions method according to EUCAST (v 11.

Epidemiology and Genetic Diversity of Colistin Nonsusceptible Nosocomial Strains from Russia for 2013-2014.

A high level of resistance to carbapenems in strains severely limits therapeutic possibilities. Colistin is the last resort drug against such strains, although the cases of resistance to this drug have become more frequent. This article presents the epidemiological features and genetic diversity of colistin nonsusceptible strains collected as part of a national multicenter epidemiological study of the antibiotic resistance of pathogens of nosocomial infections (MARATHON), which was conducted in 2013-2014 in Russia.