Studies of carbohydrate-carbohydrate-interactions by atomic force microscopy employing functionalized 4-acetylthio-butyl glucopyranosides.

By Fischer glycosylation both anomers of 4-chlorobutyl gluco-as well as galactopyranosides were obtained and transformed into the corresponding 4-acetylthio-butyl glycopyranosides. Dependent on the precursors two straightforward routes were followed to obtain the appropriate 3-O-sulfated derivatives. Unsubstituted and sulfated glucopyranosides were attached to gold surfaces a gold tips.

Enzymatic glycosylation, inhibitor design, and synthesis and formation of glyco-self assembled monolayers for simulation of recognition.

Glycosyltransferases from the albumen gland of Helix pomatia could be used in tandem mode for the chemoenzymatic synthesis of beta,1-3/beta,1-6-linked oligogalactans. By employing recombinant trans-sialidase of Trypanosoma cruzi (TcTS) the formation of a range of modified Galbeta,1-3GalNAc derivatives could be terminally alpha,2-3 sialylated. Biacore studies indicated the binding of these modified trisaccharides to myelin-associated glycoprotein (MAG).