Publications by authors named "Ethel Chitsungo"
Article Synopsis
- Peste des Petits Ruminants (PPR) is a disease common in Africa, and effective live attenuated vaccines (Nigeria 75/1 and Sungri/96 strains) are produced to combat it, but maintaining the cold chain for these vaccines is challenging.
- The study assessed the thermotolerance of various stabilizer formulations for freeze-dried and reconstituted PPR vaccines by testing their viability at different temperatures over time.
- Results indicated that the formulation with lactalbumin hydrolysate-sucrose showed the best stability, maintaining vaccine efficacy at higher temperatures, suggesting it should be the preferred stabilizer for vaccines distributed in warmer climates.
Peste des petits ruminants virus (PPRV), which is the only member of the species and belongs to the genus within the family, causes the highly contagious viral sickness "Peste des petits ruminants (PPR)." PPR is of serious economic significance for small ruminant production, particularly in Africa. Control of this critical disease depends highly on successful vaccination against the PPRV.
View Article and Find Full Text PDFContagious bovine pleuropneumonia (CBPP) is an infectious and contagious bacterial respiratory disease that affects cattle with significant economic losses to the African animal industry. The use of ELISA kits based on monoclonal antibodies (mAbs) will aid in quick and precise diagnosis of CBPP, contributing to disease control and prevention in cattle. Thus, this research aims to develop and evaluate monoclonal antibodies against CBPP (T1/44) antigen for use in ELISA kits for CBPP diagnosis.
View Article and Find Full Text PDF[This corrects the article DOI: 10.1155/2020/4236807.].
View Article and Find Full Text PDFArticle Synopsis
- Enzyme linked immunosorbent assays (ELISAs) have been designed to detect antibodies for contagious caprine pleuropneumonia (CCPP), with existing commercial kits being too expensive for many African labs.
- A new, cost-effective blocking ELISA (b-ELISA) was developed to identify antibodies against CCPP, utilizing a monoclonal antibody's epitope and positive serum samples for testing.
- The b-ELISA demonstrated high sensitivity (93%) and specificity (88%) and showed strong agreement with the commercial ELISA, indicating its effectiveness for diagnosing CCPP and monitoring during vaccination campaigns.
Peste des petits ruminants (PPR) is a contagious and economically important disease affecting production of small ruminants (i.e., sheep and goats).
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