Publications by authors named "Estrella Ruiz-Requena"
The increased vascular calcification, cardiovascular morbidity, and mortality in chronic kidney disease (CKD) patients has been associated with disturbances in mineral-bone metabolism. In order to determine markers of the vascular calcification frequently observed in these patients, blood samples of elderly male and female hemodialysis CKD patients were used to measure serum levels of: osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-κB ligand (sRANKL), and fetuin-A by enzyme immunoassay; tartrate-resistant acid phosphatase (TRACP-5b), and bone-specific alkaline phosphatase (BAP) by immunoenzymometric assay; osteocalcin (OC) by ELISA; iPTH by immunoradiometric assay; 25(OH)D(3) and 1,25(OH)(2)D(3), by I(125) radioimmunoassay; and calcium and phosphorus by photometric assay. Serum OPG, BAP, iPTH, phosphorus, and OC levels were higher and serum 25(OH)D(3), 1,25(OH)(2)D(3), and fetuin-A levels lower in both male and female CKD patients than in their respective controls.
View Article and Find Full Text PDFObjective: To study the most frequent markers of mineral-bone metabolism in young hemodialysis (HD) patients in order to detect any metabolism changes that could lead to the atherosclerosis and extravascular calcification frequently observed in chronic kidney disease (CKD) patients and estimate their potential prognostic significance.
Design And Methods: We measured serum levels of intact-PTH (iPTH), Osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-κB ligand (sRANKL), tartrate-resistant acid phosphatase (TRAP-5b), osteocalcin (Oc), bone-specific alkaline phosphatase (BAP), calcium, phosphorus, 25(OH)D(3) and 1,25 (0H)(2)D(3) in young HD patients and controls
Results: In comparison to controls, serum OPG, iPTH, BAP, phosphorus, and osteocalcin levels were higher whereas 25(OH)D(3) and 1,25(OH)(2)D(3) were lower in HD patients.
Conclusion: In conclusion, our results indicate that bone formation and resorption parameters are already altered in young HD subjects.
Aim: The aim of the study was to investigate the influence of dietary intake of commercial hydrolyzed collagen (Gelatine Royal) on bone remodeling in pre-pubertal children.
Methods: A randomized double-blind study was carried out in 60 children (9.42 +/- 1.
Objectives: To study homocysteine (Hcy) levels during post-infarction follow-up.
Methods And Results: In this study, serum Hcy levels were measured in 90 healthy individuals and in 127 patients with acute myocardial infarction (MI) on the day of infarction (day 0) and days 2, 5, 7, 9, and 11 post-infarction, comparing Hcy levels in MI patients on day 0 with their follow-up results and with levels in controls. Overall, MI patients had higher Hcy levels on day 0 versus controls.
We selected 38 patients scheduled for cholecystectomy and studied their serum concentrations of dehydroepiandrosterone (DHEA) and growth axis hormones [growth hormone/insulin-like growth factor-1 (GH/IGF-1)]. We aimed to determine whether alterations in these concentrations resulted from surgical stress or, on the contrary, preceded surgery and were themselves a cause of chronic diseases that reduce life expectancy. We measured the serum concentrations of DHEA sulfate (DHEA-S), ACTH, cortisol, human GH (hGH), IGF-1, and IGF-1 binding protein-3 (IGFBP-3) preoperatively and then 2 and 7 days after surgery; we also compared the preoperative findings with those of a healthy control group.
View Article and Find Full Text PDFBackground: Concentrations of certain substances in follicular fluid (FF) are related to fertilization outcome and early post-fertilization development. The study aim was to identify FF markers with which to predict embryo implantation potential.
Methods: Concentrations of selected hormones, cytokines and growth factors in individual FF samples obtained during assisted reproduction treatment were related with treatment outcomes.