Link between Heterotrophic Carbon Fixation and Virulence in the Porcine Lung Pathogen Actinobacillus pleuropneumoniae.

is a capnophilic pathogen of the porcine respiratory tract lacking enzymes of the oxidative branch of the tricarboxylic acid (TCA) cycle. We previously claimed that instead uses the reductive branch in order to generate energy and metabolites. Here, we show that bicarbonate and oxaloacetate supported anaerobic growth of Isotope mass spectrometry revealed heterotrophic fixation of carbon from stable isotope-labeled bicarbonate by , which was confirmed by nano-scale secondary ion mass spectrometry at a single-cell level.