Corrigendum: Geographic population structure in an outcrossing plant invasion after centuries of cultivation and recent founding events.

[This corrects the article DOI: 10.1093/aobpla/ply020.][This corrects the article DOI: 10.

Geographic population structure in an outcrossing plant invasion after centuries of cultivation and recent founding events.

Population structure and genetic diversity of invasions are the result of evolutionary processes such as natural selection, drift and founding events. Some invasions are also molded by specific human activities such as selection for cultivars and intentional introduction of desired phenotypes, which can lead to low genetic diversity in the resulting invasion. We investigated the population structure, diversity and origins of a species with both accidental and intentional introduction histories, as well as long-term selection as a cultivar.

Farnesol-mediated shift in the metabolic origin of prenyl groups used for protein prenylation in plants.

Little is known about how plant cells regulate the exchange of prenyl diphosphates between the two compartmentalized isoprenoid biosynthesis pathways. Prenylation of proteins is a suitable model to study such interactions between the plastidial methylerythritol phosphate (MEP) and the cytosolic mevalonate (MVA) pathways because prenyl moieties used to modify proteins rely on both origins. Tobacco cells expressing a prenylatable GFP were treated with specific MEP and/or MVA pathways inhibitors to block the formation of prenyl diphosphates and therefore the possibility to modify the proteins.

The effect of MEP pathway and other inhibitors on the intracellular localization of a plasma membrane-targeted, isoprenylable GFP reporter protein in tobacco BY-2 cells.

We have established an in vivo visualization system for the geranylgeranylation of proteins in a stably transformed tobacco BY-2 cell line, based on the expression of a dexamethasone-inducible GFP fused to the carboxy-terminal basic domain of the rice calmodulin CaM61, which naturally bears a CaaL geranylgeranylation motif (GFP-BD-CVIL). By using pathway-specific inhibitors it was demonstrated that inhibition of the methylerythritol phosphate (MEP) pathway with known inhibitors like oxoclomazone and fosmidomycin, as well as inhibition of the protein geranylgeranyltransferase type 1 (PGGT-1), shifted the localization of the GFP-BD-CVIL protein from the membrane to the nucleus. In contrast, the inhibition of the mevalonate (MVA) pathway with mevinolin did not affect the localization.

Two shoot-miners, Ceutorhynchus alliariae and Ceutorhynchus roberti, sharing the same fundamental niche on garlic mustard.

A combination of observational and experimental methods, in both the laboratory and field, were used to assess niche partitioning between Ceutorhynchus alliariae Brisout and C. roberti Gyllenhal (Coleoptera: Curculionidae), two coexisting shoot-boring weevils on garlic mustard, Alliaria petiolata (M. Bieb.

Herbivore resistance of invasive Fallopia species and their hybrids.

Hybridization has been proposed as a mechanism by which exotic plants can increase their invasiveness. By generating novel recombinants, hybridization may result in phenotypes that are better adapted to the new environment than their parental species. We experimentally assessed the resistance of five exotic Fallopia taxa, F.

Invasive knotweed affects native plants through allelopathy.

Premise Of Study: There is increasing evidence that many plant invaders interfere with native plants through allelopathy. This allelopathic interference may be a key mechanism of plant invasiveness. One of the most aggressive current plant invaders is the clonal knotweed hybrid Fallopia × bohemica, which often forms monocultures in its introduced range.

A role for plastids in plant protein isoprenylation.

Protein isoprenylation refers to the attachment of a C farnesyl or C geranylgeranyl moiety to a carboxyl terminal cysteine residue. Because protein isoprenyltransferases are cytosolic enzymes, it has long been assumed that the isoprenyl diphosphates used for protein isoprenylation are synthesized in the cytosol. However, in the present work, we established that the plastidial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is predominantly responsible for providing the geranylgeranyl diphosphate for protein geranylgeranylation in tobacco BY-2 cells.

The plastidial 2-C-methyl-D-erythritol 4-phosphate pathway provides the isoprenyl moiety for protein geranylgeranylation in tobacco BY-2 cells.

Protein farnesylation and geranylgeranylation are important posttranslational modifications in eukaryotic cells. We visualized in transformed Nicotiana tabacum Bright Yellow-2 (BY-2) cells the geranylgeranylation and plasma membrane localization of GFP-BD-CVIL, which consists of green fluorescent protein (GFP) fused to the C-terminal polybasic domain (BD) and CVIL isoprenylation motif from the Oryza sativa calmodulin, CaM61. Treatment with fosmidomycin (Fos) or oxoclomazone (OC), inhibitors of the plastidial 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway, caused mislocalization of the protein to the nucleus, whereas treatment with mevinolin, an inhibitor of the cytosolic mevalonate pathway, did not.

Demographic models inform selection of biocontrol agents for garlic mustard (Alliaria petiolata).

Nonindigenous invasive plants pose a major threat to natural communities worldwide. Biological control of weeds via selected introduction of their natural enemies can affect control over large spatial areas but also risk nontarget effects. To maximize effectiveness while minimizing risk, weed biocontrol programs should introduce the minimum number of host-specific natural enemies necessary to control an invasive nonindigenous plant.

A review of tobacco BY-2 cells as an excellent system to study the synthesis and function of sterols and other isoprenoids.

In plants, two pathways are utilized for the synthesis of isopentenyl diphosphate (IPP), the universal precursor for isoprenoid biosynthesis. In this paper we review findings and observations made primarily with tobacco BY-2 cells (TBY-2), which have proven to be an excellent system in which to study the two biosynthetic pathways. A major advantage of these cells as an experimental system is their ability to readily take up specific inhibitors and stably- and/or radiolabeled precursors.