Interactions between type of culture medium and addition of serum on development and gene expression of bovine blastocysts produced in vitro.

Serum is commonly added to culture medium to improve the production of bovine embryos in vitro. The main goals were to verify the actions of serum to increase blastocyst yield and test the hypothesis that fetal bovine serum alters blastocyst gene expression in a manner that could affect competence to establish pregnancy and dysregulate fetal development. Media used were synthetic oviduct fluid medium bovine embryo 2 (SOF-BE2) and a commercial medium from IVF Biosciences termed here as IVFB.

TEAD3 and TEAD4 play overlapping role in bovine preimplantation development.

In Brief: Transcription factors are crucial for lineage specification in the preimplantation development of mammals. This research shows that TEAD3 and TEAD4 play important and overlapping functions in directing trophectoderm fate decisions during this developmental stage in cattle.

Abstract: During mammalian preimplantation development, the transition from morula to blastocyst is a critical biological event.

Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.

In livestock breeding, oocyte cryopreservation is crucial for preserving and transferring superior genetic traits. This study was conducted to examine the additional effect of melatonin to maturation and vitrification media on the in vitro developmental capacity, mitochondrial distribution, and intensity of buffalo oocytes. The study involved obtaining ovaries from a slaughterhouse and conducting two phases.