Finding the DNA of the human immune deficiency virus (HIV) with simple and sensitive detection is the main challenge in early diagnosis of AIDS. Herein, two-point separation strategies based on the colorimetric and fluorescence are introduced. The naked-eye qualitative and semiquantitative colorimetric, and also accuracy fluorescence quantification of HIV-1 DNA were applied using label-free NiFeO@UiO-66 nanozyme with both functions of peroxidase-mimetic like and emitting fluorescence.
View Article and Find Full Text PDFWe investigate the linear and nonlinear optical property of Morin (MN) at different concentration (1 × 10-6 and 5 × 10-6 M) within AOT reversed micelle prepared by water-in-decane microemulsion having a constant molar ratio of water-to-surfactant molecules of 40 (W = [H2O]/[AOT] = 40) as well as the function of mass fraction of nano-droplet (MFD) values of 0.01,0.04, 0.
View Article and Find Full Text PDFNew trans-AB-porphyrins substituted at phenyl positions were synthesized from 4-methylphthalic acid as a starting material through sequential multistep reactions. These macrocycles were characterized by H NMR, C NMR, F NMR, H-H COSY NMR, and MALDI-TOF mass spectrometry. Computational studies were performed on the porphyrins to investigate various factors such as structural features, electronic energy, energy gaps, and aromaticity.
View Article and Find Full Text PDFBackground And Objectives: The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by leaf extract against and .
Materials And Methods: Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and leaf extract.
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable.
View Article and Find Full Text PDFA new, simple, and fast method for preconcentration and determination of trace amount of lead from biological samples was developed by modified silver nanoparticle-based solid-phase extraction technique and graphite furnace atomic absorption spectrometry. In this study, morin was used as a complexing agent. Some factors influencing the recovery of lead including pH, sample flow rate, type, flow rate, and least amount of the eluent for elution of the lead from silver nanoparticles were studied and optimized.
View Article and Find Full Text PDFIn this study is proposed a pre-concentration procedure using molecular imprinted polymer for the determination of copper in food samples. The copper imprinted polymer was prepared by free radical solution polymerization in a tube containing copper, morin, 4-vinylpyridine as a functional monomer, ethyleneglycoldimethacrylate as a cross-linking monomer, and 2,2'-azobisisobutyronitrile as an initiator. The polymer particles synthesized both before and after leaching have been characterized by infrared and X-ray diffraction studies.
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