What are the effects of whole blood storage conditions on comet assay in terms of DNA damage and repair?

The comet assay is a rapid, simple and sensitive method for the detection of DNA damage and repair at the level of individual cells, with a wide range of applications in human biomonitoring and molecular epidemiology. It is common practice to perform the comet assay on fresh samples to preserve the integrity of the DNA and to obtain reliable results, which is why most published studies have been designed using fresh blood samples. There are limitations associated with the use of fresh samples for this assay and the need for appropriate storage for some studies.

Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry.

Various technical methodologies are required to accurately detect substances of different chemical and pharmacological properties in biological samples, which are increasing in number and variety daily. Therefore, laboratories where many samples and different factors are analyzed simultaneously need methods with easy sample preparation, short analysis times and low analysis costs. In this study, the objective was to scan substances susceptible to chemical degradation, amenable to analysis without hydrolysis, and exhibiting short-term stability by employing a straightforward, expeditious, and cost-efficient method.