Marine Natural Compound (Neviotin A) Displays Anticancer Efficacy by Triggering Transcriptomic Alterations and Cell Death in MCF-7 Cells.

We investigated the anticancer mechanism of a chloroform extract of marine sponge () (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using MTT and neutral red uptake (NRU) assays showed that sample C exposure decreased the proliferation of cells. Flow cytometric data exhibited reactive oxygen species (ROS), nitric oxide (NO), dysfunction of mitochondrial potential, and apoptosis in sample C-treated MCF-7 cells.

CuO Nanoparticle-Mediated Seed Priming Improves Physio-Biochemical and Enzymatic Activities of .

The use of nanoparticles (NPs) in agricultural fields has risen to a level where people are considering NPs as an alternative to commercial fertilizers. The input of copper oxide NPs (CuO NPs) as seed primers was investigated in this study, and the growth indices of such as phenotypic parameters, photosynthetic attributes, and biochemical parameters were measured during maximum vegetative growth stage, i.e.

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in L.

Efficient methods for callus induction and the high-frequency plant regeneration of L. were established, and the phytochemical potential and antioxidant activity of a donor plant, ex-vitro-established micropropagated plants, and callus were also studied. Yellowish-green callus was induced with a frequency of 97.

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Roxb.

Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood.

High-Frequency Plant Regeneration, Genetic Uniformity, and Flow Cytometric Analysis of Regenerants in L.

L., an evergreen shrub in the citrus family, is well-known around the world for its essential oils and variety of bioactivities, indicating its potential medicinal applications. In this study, we investigated the effect of different culture conditions, including plant growth regulators, media types, pH of the medium, and carbon sources, on in vitro regeneration from nodal explants of .

Integrated Analysis of Transcriptional and Metabolic Variations in Salt-Treated Desert Plants.

(Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of plants to salt stress are still poorly understood.

Tissue-specific analysis of L. transcriptome revealed potential regulatory roles of lncRNAs.

Long non-coding RNAs (lncRNAs) play pivot roles in regulating mRNA expression in eukaryotic organisms without coding any proteins. In the current study, a comprehensive analysis of 260 published RNA-Seq datasets collected from different tissues (fruits, leaves, stems, and roots) of L. was performed to discover potential lncRNAs.

New Approach for Using of L. and L. Essential Oils as Wood-Biofungicides: GC-MS, SEM, and MNDO Quantum Chemical Studies.

Background: Fungi growing on wood cause deterioration of stored food materials or discoloration of the wood itself, and the search for new and safe bioagents is recently needed.

Methods: Essential oils (EOs) from aerial parts from L. and L.

Induction of somatic embryogenesis in L. and analysis of regenerants using ISSR-PCR and flow cytometer.

A new and simple protocol has been developed and standardized for direct somatic embryogenesis and plant regeneration from aseptic seedlings derived from immature seeds. Depending on the age of immature seeds and nutrient media, in vitro occurrence of embryogenesis and the number of embryos from each seedling have varied greatly. The largest number of somatic embryos, producing 12.

Genome-wide transcriptome variation landscape in Ruta chalepensis organs revealed potential genes responsible for rutin biosynthesis.

Ruta chalepensis L., most commonly known as 'fringed rue,' is an excellent and valuable bioactive plant that produces a range of complex flavonoids, of which rutin is the major compound present in this plant of great pharmaceutical and medicinal significance. The present study is a pioneering attempt to examine the changes in the transcriptomic landscape of leaf, stem, and root tissues and correlate this with rutin quantity in each tissue in order to identify the candidate genes responsible for rutin biosynthesis and to increase genomic resources in fringed rue.

Role of nanocatalyst in the treatment of organochlorine compounds - A review.

Since a few centuries ago, organochlorine compounds (OCs) become one of the threatened contaminants in the world. Due to the lipophilic and hydrophobic properties, OCs always discover in fat or lipid layers through bioaccumulation and biomagnification. The OCs are able to retain in soil, sediment and water for long time as it is volatile, OCs will evaporate from soil and condense in water easily and frequently, which pollute the shelter of aquatic life and it affects the function of organs and damage system in human body.

Remote sensing of 10 years changes in the vegetation cover of the northwestern coastal land of Red Sea, Saudi Arabia.

Accurate and up to date land use and land cover (LU/LC) changes information is the main source to understanding and assessing the environmental outcomes of such changes and is important for development plans. Thus, this study quantified the outlines of land cover variation of 10-years in the northwestern costal land of the Red Sea, Saudi Arabia. Two different supervised classification algorithms are visualized and evaluated to preparing a policy recommendation for the proper improvements towards better determining the tendency and the proportion of the vegetation cover changes.

sp. ZB is a cell factory for the production of gibberellic acid using a cheap substrate in solid state fermentation.

Gibberellic acid from the fungi has been widely used in agriculture. In this study, more than 20 fungal isolates were screened and sp. ZB shown to produce more gibberellic acid than other fungal isolates.

Comparative Analysis between Wild and Cultivated Cucumbers Reveals Transcriptional Changes during Domestication Process.

The cultivated cucumber ( L.) was reported to have been developed from a wild cucumber ( Chakrav.), nevertheless, these two organisms exhibit noteworthy differences.

Effects of 4-CPPU on in vitro multiplication and sustainable generation of L. 'White Butterfly'.

There are more than nine thousand cultivars of L., with a series of flowers with shapes, colors and new cultivars continues as generated through both traditional and modern breeding techniques. In this study, advanced biotech methods of in vitro culture have been used to identify a technique for the efficient mass multiplication of 'White Butterfly', using phenyl urea, (4-CPPU).

Antioxidant, Hypoglycemic, and Neurobehavioral Effects of a Leaf Extract of on Autoimmune Diabetic Mice.

Diabetes mellitus (DM) is a metabolic disease that can affect the central nervous system and behavioral traits in animals. Streptozotocin-induced diabetes is considered an autoimmune disease. The aim of the current study was to determine whether supplementation with the alcoholic extract of leaves could improve diabetes-associated pathological changes.

Assessment of the Impact of Different Treatments on the Technological and Antifungal Properties of Papyrus ( L.) Sheets.

In the present work, sheets of Papyrus ( L.), manufactured by lamination from strips pre-treated with different treatments, were evaluated for their technological and fungal infestation properties ( AFl375, Ani245 and Cgl311). The results showed that the highest values of tensile strength, tear strength, burst index and double-fold number were observed in papyrus sheets produced from strips treated with nano-cellulose (0.

Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists.

Plants are sessile organisms and, in order to defend themselves against exogenous (a)biotic constraints, they synthesize an array of secondary metabolites which have important physiological and ecological effects. Plant secondary metabolites can be classified into four major classes: terpenoids, phenolic compounds, alkaloids and sulphur-containing compounds. These phytochemicals can be antimicrobial, act as attractants/repellents, or as deterrents against herbivores.

Bioactivities of Traditional Medicinal Plants in Alexandria.

In traditional folklore, medicinal herbs play a vital role in the prevention and treatment of microbial diseases. In the present study, the phenolic profiles of the medicinal plants L., L.

Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt.

Background: Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D.

Pendimethalin induces oxidative stress, DNA damage, and mitochondrial dysfunction to trigger apoptosis in human lymphocytes and rat bone-marrow cells.

Pendimethalin (PM) is a dinitroaniline herbicide extensively applied against the annual grasses and broad-leaved weeds. There is no report available on PM-induced low-dose genotoxicity in human primary cells and in vivo test models. Such data gap has prompted us to evaluate the genotoxic potential of PM in human lymphocytes and rats.

Efficient and reproducible regeneration of and assessment genetic uniformity using flow cytometry and SPAR methods.

In the present study, we develop an efficient and reproducible regeneration system for two cultivars , Jamila and Tomaland of L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.

Titanium dioxide nanoparticles preferentially bind in subdomains IB, IIA of HSA and minor groove of DNA.

Titanium dioxide nanoparticles (TiO-NPs) interaction with human serum albumin (HSA) and DNA was studied by UV-visible spectroscopy, spectrofluorescence, circular dichroism (CD), and transmission electron microscopy (TEM) to analyze the binding parameters and protein corona formation. TEM revealed protein corona formation on TiO-NPs surface due to adsorption of HSA. Intrinsic fluorescence quenching data suggested significant binding of TiO-NPs (avg.