Functionally important components of the transcription elongation complex involved in Rho-dependent termination.

Bacterial transcription terminator, Rho is an RNA-dependent ATPase that terminates transcription. Several structures of pre-termination complexes of the Rho-transcription elongation complex (EC) revealed a static picture of components of the EC that come close to the nascent RNA-bound Rho, where many of the residues of EC reside ≤ 10 Å from the Rho residues. However, the in vitro-formed Rho-EC complexes do not reveal the in vivo Rho-EC dynamic interaction patterns during the termination process.

Clinical spectrum of bilateral microspherophakia and risk factors associated with poor visual outcomes following lensectomy surgery - A multicentric study.

Purpose: To study the clinical profile of patients with microspherophakia and the factors associated with poor vision following lensectomy surgery.

Methods: A multicenter, retrospective, cross-sectional analysis was conducted from January 2010 to June 2022 on patients diagnosed with microspherophakia.

Results: A total of 102 eyes from 51 patients were enrolled, of whom 24 (47.

Perivascular Invasion: A Promising Prognostic Parameter for Breast Cancer.

Objective: Angiotropism/perivascular invasion (PVI) is an emerging topic in various types of cancer, with studies primarily focusing on melanoma. However, limited data are available on the significance of PVI in breast cancer. This study aimed to assess the prognostic significance of PVI in breast cancer and its correlation with traditional clinicopathological prognostic parameters.

Ultrastructural imaging biomarkers in diabetic macular edema: A major review.

Diabetic macular edema (DME) is a vision-threatening complication of diabetic retinopathy and causes significant morbidity in patients. Anti-vascular endothelial growth factor (VEGF) agents are the mainstay of treatment for DME, with steroid implants being used for the treatment of anti-VEGF resistant eyes. Over the years, several classification systems have been devised to describe the patterns of DME using optical coherence tomography (OCT).

Cerebral collateral flow state in acute ischemic stroke correlates with clinical functional outcomes in non-thrombectomy patients.

Introduction: Hypoperfusion index ratio (HIR) measured by computerized tomography perfusion (CTP) has been shown to predict collateral flow state in acute ischemic stroke (AIS). Low HIR (<0.4) is indicative of good collateral flow state.

Bulk-like structural, magnetic and optical properties of (111)- and (001)-NiO thin films.

We have grown (111)- and (001)-oriented NiO thin films on (0001)-Sapphire and (001)-MgO substrates using pulsed laser deposition (PLD), respectively. DC magnetic susceptibility measurements underline that the Néel temperatures of the samples are beyond room-temperature. This is further confirmed by the presence of two-magnon Raman scattering modes in these films in ambient conditions.

Effects of 1-methylcyclopropene, methyl jasmonate and salicylic acid on physicochemical properties and wooliness of nectarine fruit during cold storage.

Background: Owing to its high perishability, the market life of nectarine fruit is very short. Cold storage is a principal approach to limit post-harvest quality loss in nectarines. The objective of this research was to evaluate the impact of postharvest methyl jasmonate (MeJA), salicylic acid (SA) and 1-methylcyclopropene (1-MCP) on quality properties of nectarine fruit, specifically weight loss, firmness, phenolics and antioxidant activity, following cold storage and subsequent shelf life.

Effects of virtual reality exercise on pain, joint motion, and quality of life in patients with frozen shoulder: a randomized controlled study.

Background: Restriction of shoulder movements in frozen shoulder may negatively affect individual's daily living activities. The effect of the virtual reality (VR)-based exercise program, which is an innovative application and has been used in the field of physiotherapy in recent years, is unknown on patients diagnosed with frozen shoulder.

Objective: This study was conducted to determine the effects of a VR-based exercise program provided to patients with frozen shoulder on their pain, joint motion, and quality of life.

Constrictive pericarditis following epicardial pacemaker implant: a diagnostic dilemma.

Constrictive pericarditis is a rare complication after cardiac surgery. It is mostly seen in adults. We report a case of constrictive pericarditis in a 3-year-old child with right ventricular dysfunction after permanent pacemaker implantation during infancy for congenital complete heart block.

In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots.

Background: Nitrate (NO) is one of the two major forms of inorganic nitrogen absorbed by plant roots, and the tissue nitrate concentration in roots is considered important for optimizing developmental programs. Technologies to quantify the expression levels of nitrate transporters and assimilating enzymes at the cellular level have improved drastically in the past decade. However, a technological gap remains for detecting nitrate at a high spatial resolution.

Single-Molecule Tracking dataset for histone H3 (hht1) from live and fixed cells of Schizosaccharomyces pombe.

Single-molecule tracking provides direct real-time measurements of protein dynamics in live cells with high spatiotemporal resolution. In the last decade, this method has been adopted by several labs to understand the dynamics of DNA replication, transcription, telomerase, chromatin remodeling, etc. in a variety of model systems (bacteria, yeast S.

Dynamical phase transitions in the XY model: A Monte Carlo and mean-field-theory study.

We investigate the dynamical phases and phase transitions arising in a classical two-dimensional anisotropic XY model under the influence of a periodically driven temporal external magnetic field in the form of a symmetric square wave. We use a combination of finite temperature classical Monte Carlo simulation, implemented within a CPU+GPU paradigm, utilizing local dynamics provided by the Glauber algorithm and a phenomenological equation-of-motion approach based on relaxational dynamics governed by the time-dependent free energy within a mean-field approximation to study the model. We investigate several parameter regimes of the variables (magnetic field, anisotropy, and the external drive frequency) that influence the anisotropic XY system.

Critical Role of Water beyond the Media to Maintain Protein Stability and Activity in Hydrated Deep Eutectic Solvent.

Hydrated deep eutectic solvents (DESs) are recognized for their potential in biocatalysis due to their tunability, biocompatibility, greenness, and ability to keep protein stable and active. However, the mechanisms governing enzyme stability and activity in DES remain poorly understood. Herein, using bromelain as the model enzyme and acetamide (0.

Mission SpaceX CRS-19 RRRM-1 space flight induced skin genomic plasticity via an epigenetic trigger.

Genomic plasticity helps adapt to extreme environmental conditions. We tested the hypothesis that exposure to space environment (ESE) impacts the epigenome inducing genomic plasticity. Murine skin samples from the Rodent Research Reference Mission-1 were procured from the International Space Station (ISS) National Laboratory.

Efficacy and Safety of CT-P13 as First- and Second-Line Treatment in Patients with Ankylosing Spondylitis.

CT-P13 is a biosimilar version of infliximab, a monoclonal antibody. In individuals with ankylosing spondylitis (AS), CT-P13 has been shown to be effective and to have a well-tolerated safety profile. The aim of this study was to evaluate the long-term drug persistence, safety, and efficacy of infliximab biosimilar CT-P13 in patients with AS undergoing first-line (1st-line) and later (≥2nd-line) treatment in clinical practice.

Comparison of Biomarkers Playing a Role in Pterygium Development in Pterygium and Recurrent Pterygium Tissues.

Pterygium is a nonneoplastic elastotic degeneration characterized by subepithelial growth. It manifests as an ocular lesion originating from the bulbar conjunctiva, extending to the corneal surface, and reaching the visual axis in some cases. Although the exact cause is unknown, prolonged exposure to ultraviolet radiation is considered the most significant contributing factor.

Effect of graphene oxide on the biaxial flexural strength and translucency of ultrathin monolithic zirconia.

Purpose: The purpose of this study was to investigate the influence of graphene oxide (GO) and hydrothermal aging on the biaxial flexural strength (BFS) and translucency (TP) of ultrathin monolithic zirconia with different yttria concentrations.

Methods: Disc shaped specimens (n = 120) were milled using zirconia blocks (YZ-HT [HT], YZ-ST [ST], and YZ-XT [XT]) with a diameter of 15.0 mm and a thickness of 0.

Dicle University Hospital's hospital disaster plan and emergency service management: Kahramanmaraş earthquake experience.

Background: Hospitals, being establishments with varying functions and capacities, must have disaster plans that reduce vulnerability, ensure the continuity, and possibly increase the capacity of healthcare services; these measures are crucial for reducing mortality and facilitating the normalization of life after a disaster. In this study, the aim was to analyze the operational process of the disaster management plan at Dicle University Faculty of Medicine Hospital following the earthquake in Kahramanmaraş on 6 February 2023.

Methods: This is a retrospective observational study.

Deciphering the Comprehensive Structure-Activity Relationship of Sunshinamide for Breast Cancer Therapy through Dual Modulation of Apoptotic and Ferroptotic Pathways via TrxR1 and Gpx4 Inhibition.

Sunshinamide, a cyclodepsipeptide, has demonstrated significant potential in inhibiting cancer cell proliferation. Our prior research established the total synthesis and anticancer properties of sunshinamide. However, a deeper understanding of the structure-activity relationship (SAR) of sunshinamide remained imperative.

FVIIa-PAR2 SIGNALING FACILITATES IMMUNE ESCAPE BY REDUCING PHAGOCYTIC POTENTIAL OF MACROPHAGES IN BREAST CANCER.

Background: Treatment of breast cancers with immunotherapy has so far achieved limited success. Traditional immunotherapies focusing on cytotoxic T cells have attained modest success, while the approval of phagocytic checkpoint blockers is still pending. Coagulation proteases are crucial elements pertaining to cancer growth and proliferation, but their relevance in altering the immunological topography in tumours remain largely uncharted.

Affective modulation of emotional reactivity in euthymic patients with bipolar disorder.

Background: Affective modulation of startle reflex (AMSR) is a widely used experimental tool for assessing emotional reactivity. Previous research has shown inconsistent findings of emotional reactivity in amplitude in bipolar patients (BP). This study examined emotional reactivity (ER) in euthymic BP compared to healthy controls using both subjective and objective measures.

Cataractous Eye Protein Isolate Stabilized Gold Nanoparticles Prevent Their Ethanol-Induced Aggregation.

Due to their distinctive optical, electrical, and catalytic characteristics, gold nanoparticles (AuNPs) have found increasing use for a wide range of applications, including biomedicine and catalysis. Inherent agglomeration propensities impair their functional qualities, stability, and biocompatibility. This work investigates the potential applications of the cataractous eye protein isolate (CEPI), a waste product rich in proteins from cataract surgery, as a novel AuNP stabilizing agent.