Recent advances in the multifaceted functions of Cys2/His2-type zinc finger proteins in plant growth, development, and stress responses.

Recent research has highlighted the importance of Cys2/His2-type zinc finger proteins (C2H2-ZFPs) in plant growth and in responses to various stressors, and the complex structures of C2H2-ZFP networks and the molecular mechanisms underlying their responses to stress have received considerable attention. Here, we review the structural characteristics and classification of C2H2-ZFPs, and consider recent research advances in their functions. We systematically introduce the roles of these proteins across diverse aspects of plant biology, encompassing growth and development, and responses to biotic and abiotic stresses, and in doing so hope to lay the foundations for further functional studies of C2H2-ZFPs in the future.

Yarrowia lipolytica increased the activities of disease defense related enzymes and anti-fungal compounds in asparagus (Asparagus officinalis).

Fungal diseases pose significant threats to the production of asparagus, resulting in economic losses and decreased crop quality. The potential of the yeast Yarrowia lipolytica as a biocontrol agent against Fusarium proliferatum, a common pathogen of asparagus, was investigated in this study. The effects of Y.

A transcriptome profile of Wickerhamomyces anomalus incubated with chitosan revealed dynamic changes in gene expression and metabolic pathways.

Previous studies have shown that Wickerhamomyces anomalus can control postharvest diseases of fruits and incubation of the yeast with chitosan can improve its efficiency. In this study, transcriptome study was conducted to determine molecular mechanisms involved in the yeast-chitosan interaction. The bioinformatics analysis of the RNA-seq data confirmed that incubating W.

Infection of postharvest pear by Penicillium expansum is facilitated by the glycoside hydrolase (eglB) gene.

The primary reason for postharvest loss is blue mold disease which is mainly caused by Penicillium expansum. Strategies for disease control greatly depend on the understanding of mechanisms of pathogen-fruit interaction. A member of the glycoside hydrolase family, β-glucosidase 1b (eglB), in P.

Biotechnological and Biocontrol Approaches for Mitigating Postharvest Diseases Caused by Fungal Pathogens and Their Mycotoxins in Fruits: A Review.

Postharvest diseases caused by fungal pathogens are significant contributors to the postharvest losses of fruits. Moreover, some fungal pathogens produce mycotoxins, which further compromise the safety and quality of fruits. In this review, the potential of biotechnological and biocontrol approaches for mitigating postharvest diseases and mycotoxins in fruits is explored.

A First Expression, Purification and Characterization of Endo-β-1,3-Glucanase from .

β-1,3-glucanase plays an important role in the biodegradation, reconstruction, and development of β-1,3-glucan. An endo-β-1,3-glucanase which was encoded by was expressed, purified and characterized from for the first time. The gene was amplified and transformed into the competent cells of Rosetta strain with the help of the pET-30a cloning vector.

Biodegradation of patulin in fresh pear juice by an aldo-keto reductase from Meyerozyma guilliermondii.

Bio-enzymes have shown broad application prospects in controlling mycotoxins due to their strong specificity, fast reaction rate and mild reaction conditions. However, the number of enzymes isolated, purified and characterized to degrade patulin (PAT) is limited. We expressed an aldo-keto reductase (MgAKR) from Meyerozyma guilliermondii in Escherichia coli.

Ultrastructural observation and transcriptome analysis provide insights into mechanisms of Penicillium expansum invading apple wounds.

Penicillium expansum is a pathogen causing enormous postharvest losses of fruits, especially apples. In this study, we first investigated the morphological changes of P. expansum within apple wounds during infectious process by microscopic observation.

Proteomic Analysis of Apple Response to Infection Based on Label-Free and Parallel Reaction Monitoring Techniques.

Blue mold, caused by , is the most destructive fungal disease of apples and causes great losses during the post-harvest storage of the fruit. Although some apple cultivars are resistant to there has been little information on the molecular mechanism of resistance. In this study, differential proteomic analysis was performed on apple samples infected and uninfected with .

The necrosis-inducing protein (NIP) gene contributes to Penicillium expansum virulence during postharvest pear infection.

Penicillium expansum is the causative fungus of blue mold decay in postharvest pears resulting in substantial economic losses. Investigating P. expansum-pear fruit interactions is necessary to help develop P.

Efficacy of the Yeast in Biocontrol of Gray Mold Decay of Tomatoes and Study of the Mechanisms Involved.

Gray mold decay is a widespread postharvest disease in tomato that results from infection by the pathogen , leading to huge economic losses. The objective of this study was to select the most effective antagonistic yeast to control tomato gray mold from six potential biocontrol agents and to investigate the possible control mechanism. The results showed that the yeast was the most effective in inhibiting among the six strains both in vivo and in vitro on tomato, with a colony diameter of 11 mm, a decay diameter of 20 mm, and the lowest decay incidence (53%)-values significantly smaller and lower than the values recorded for the control group and the other yeasts.

Recent advances in infection mechanisms and current methods in controlling in postharvest apples.

One of the most significant challenges associated with postharvest apple deterioration is the blue mold caused by , which leads to considerable economic losses to apple production industries. Apple fruits are susceptible to mold infection owing to their high nutrient and water content, and current physical control methods can delay but cannot completely inhibit growth. Biological control methods present promising alternatives; however, they are not always cost effective and have application restrictions.

Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease.

To study the mechanism by which induced with chitosan (1% w/v) controls blue mold disease in table grapes caused by , this study evaluated alterations in three yeast enzymatic activities. The changes in the five primary disease defense-related enzymes and two non-enzyme activities of table grapes were assayed. The results of the study showed that chitosan (1% w/v) significantly increased the yeast β-1,3-glucanase, catalase (CAT), and malondialdehyde (MDA) activities.

Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to Induced with Chitosan.

Transcriptome analysis (TA) was conducted to characterize the transcriptome changes in postharvest disease-related genes of table grapes following treatment with induced with chitosan (1% /). In the current study, the difference in the gene expression of table grapes after treatment with induced with chitosan and that of a control group was compared 72 h post-inoculation. The study revealed that postharvest treatment of table grapes with induced with chitosan could up-regulate genes that have a pivotal role in the fruit's disease defense.

Biological control as an alternative to synthetic fungicides for the management of grey and blue mould diseases of table grapes: a review.

Grey and blue mould diseases are among the most important diseases of grapes worldwide. They are causing extensive decay in postharvest grapes. Chemical fungicides remain the primary treatment for managing these diseases.