The clinical relevance of excisional margins after large loop excision of the transformation zone for the persistence of cervical dysplasia.

Objective: A new guideline on population-screening cervical cytology was introduced to improve diagnosis and management of (pre-)malignant cervical lesions. Subsequently, more colposcopies and more large loop excision of the transformation zone (LLETZ) were performed. There is little information about the relevance of positive margins for cervical intraepithelial neoplasia (CIN) after LLETZ.

Feasibility of endometrial sampling by vaginal tampons in women with Lynch syndrome.

Background: Endometrial sampling for the surveillance of women with Lynch syndrome is an invasive and painful procedure. The aim of this study was to evaluate the feasibility of a less invasive procedure of collecting vital cells by vaginal tampons.

Methods: This was a prospective feasibility study of women scheduled to undergo annual gynecological surveillance, including endometrial sampling.

Altered bile composition after liver transplantation is associated with the development of nonanastomotic biliary strictures.

Background/aims: Nonanastomotic biliary strictures are troublesome complications after liver transplantation. The pathogenesis of NAS is not completely clear, but experimental studies suggest that bile salt toxicity is involved.

Methods: In one hundred and eleven adult liver transplants, bile samples were collected daily posttransplantation for determination of bile composition.

Hepatic expression of ABC transporters G5 and G8 does not correlate with biliary cholesterol secretion in liver transplant patients.

The adenosine triphosphate (ATP)-binding cassette (ABC)-transporters ABCG5 and ABCG8 have been shown to mediate hepatic and intestinal excretion of cholesterol. In various (genetically modified) murine models, a strong relationship was found between hepatic expression of ABCG5/ABCG8 and biliary cholesterol content. Our study aimed to relate levels of hepatic expression of ABCG5 and ABCG8 to biliary excretion of cholesterol in man.

Expression of heme oxygenase-1 in human livers before transplantation correlates with graft injury and function after transplantation.

Upregulation of heme oxygenase-1 (HO-1) has been proposed as an adaptive mechanism protecting against ischemia/reperfusion (I/R) injury. We investigated HO-1 expression in 38 human liver transplants and correlated this with I/R injury and graft function. Before transplantation, median HO-1 mRNA levels were 3.

Rapid increase of bile salt secretion is associated with bile duct injury after human liver transplantation.

Background/aims: Biliary strictures are a serious cause of morbidity after liver transplantation. We have studied the role of altered bile composition as a mechanism of bile duct injury after human liver transplantation.

Methods: In 28 liver transplant recipients, bile samples were collected daily posttransplantation for determination of bile composition.

Liver transplantation with preservation of the inferior vena cava. A comparison of conventional and piggyback techniques in adults.

The aim of this study is to analyse a single centre's experience with two techniques of liver transplantation (OLT), conventional (CON-OLT) and piggyback (PB-ES), and to compare outcome in terms of survival, morbidity, mortality and post-operative liver function as well as operative characteristics. A consecutive series (1994-2000) of 167 adult primary OLT were analysed. Ninety-six patients had CON-OLT and 71 patients had PB-ES.