Corrigendum to "Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression" [Synth Syst Biotechnol 2 (4) (2017) 295-301].

[This corrects the article DOI: 10.1016/j.synbio.

Corrigendum to "YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in ".

[This corrects the article DOI: 10.1016/j.meteno.

Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression.

Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering. Along the way, combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic circuits. In this report, we present an efficient evolutionary approach to build a range of regulatory control elements.

YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in .

Effective metabolic engineering of microorganisms relies on balanced expression of both heterologous and endogenous genes to channel metabolic flux towards products of interest while achieving reasonable biomass buildup. To facilitate combinatorial pathway engineering and facile genetic operation, we engineered a set of modular cloning vectors compatible with BioBrick standards, called YaliBricks, to allow for rapid assembly of multigene pathways with customized genetic control elements (promoters, intronic sequences and terminators) in the oleaginous yeast . We established a sensitive luciferase reporter and characterized a set of 12 native promoters to expand the oleaginous yeast genetic toolbox for transcriptional fine-tuning.