A Simple and User-Friendly Method for High-Quality Preparation of Pollen Grains for Scanning Electron Microscopy (SEM).

Pollen is becoming an increasingly important subject for molecular researchers in genetic engineering, plant breeding, and environmental monitoring. To broaden the scope of these studies, it is essential to develop accessible methods for scientists who are not specialized in palynology. The article presents a simplified technical procedure for preparing pollen grains for scanning electron microscopy (SEM).

Automating physical intuition in nonlinear fiber optics with unsupervised dominant balance search.

Identifying the underlying processes that locally dominate physical interactions is the key to understanding nonlinear dynamics. Machine-learning techniques have recently been shown to be highly promising in automating the search for dominant physics, adding important insights that complement analytical methods and empirical intuition. Here we apply a fully unsupervised approach to the search for dominant balance during nonlinear and dispersive propagation in an optical fiber and show that we can algorithmically identify dominant interactions in cases of optical wavebreaking, soliton fission, dispersive wave generation, and Raman soliton emergence.

Isolated Medial Platysmaplasty and Harmonization of the Lower Third of Face with Local Fat Autograft: A Case Report.

One of the most common complaints of patients seeking plastic surgery is the presence of age-related changes in the lower third of the face. These often include vertical platysmal bands, a double chin, Venus rings, the absence of the cervico-mental angle, hypertonicity of the muscles in the mental area, and insufficient projection of the chin. The development and implementation of less-traumatic methods of correction of the lower third of the face and harmonization of the projection of the cervico-mental region remain relevant.

Microsurgical Clip Reconstruction of a True Posterior Communicating Artery Aneurysm.

Approximately 25% of intracranial aneurysms originate at the internal carotid artery and posterior communicating artery (PCoA) junction. In contrast to typical PCoA aneurysms, which are usually saccular, a subset known as true PCoA aneurysms arise directly from the PCoA. These represent about 1.

CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale.

The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.

Gaston Ramon (to Centenary of the discovery of anatoxins).

In the history of science, there are great scientists who, without being physicians, wrote golden pages in the History of Medicine. Such was Louis Pasteur, founder of scientific microbiology and immunology. Such was his follower Gaston Ramon (1886-1963), French veterinarian and immunologist who created 100 years ago first anatoxin for active prevention of diphtheria and later tetanus and thus opened era of anatoxins (toxoids) - vaccines based on toxin molecule devoid of toxic properties, but preserving immunogenicity and antigenic specificity.

Erratum: Boiss. repeatome characterized using low-coverage NGS as a source of new FISH markers: application in phylogenetic studies of the Triticeae.

[This corrects the article DOI: 10.3389/fpls.2022.

Analysis of interaction dynamics and rogue wave localization in modulation instability using data-driven dominant balance.

We analyze the dynamics of modulation instability in optical fiber (or any other nonlinear Schrödinger equation system) using the machine-learning technique of data-driven dominant balance. We aim to automate the identification of which particular physical processes drive propagation in different regimes, a task usually performed using intuition and comparison with asymptotic limits. We first apply the method to interpret known analytic results describing Akhmediev breather, Kuznetsov-Ma, and Peregrine soliton (rogue wave) structures, and show how we can automatically distinguish regions of dominant nonlinear propagation from regions where nonlinearity and dispersion combine to drive the observed spatio-temporal localization.

Anterior transpetrosal (Kawase) approach for petroclival meningioma with trigeminal neuralgia: case report and literature review.

The authors present a patient with petroclival meningioma complicated by trigeminal neuralgia. Resection of tumor via anterior transpetrosal approach with microvascular decompression of the trigeminal nerve was performed. A 48-year-old female patient presented with left-sided (V1-V2) trigeminal neuralgia.

The Control of the Crossover Localization in Allium.

Meiotic crossovers/chiasmata are not randomly distributed and strictly controlled. The mechanisms behind crossover (CO) patterning remain largely unknown. In , as in the vast majority of plants and animals, COs predominantly occur in the distal 2/3 of the chromosome arm, while in they are strictly localized in the proximal region.

Hidden Pitfalls of Using Onion Pollen in Molecular Research.

There is little information on the use of pollen in molecular research, despite the increased interest in genome editing by pollen-mediated transformation. This paper presents an essential toolbox of technical procedures and observations for molecular studies on onion ( L.) pollen.

How I do it: The microsurgical clipping of the giant paraclinoid aneurysm using the retrograde suction decompression technique.

Background: Paraclinoid aneurysms account for 5.4% of all intracranial aneurysms. Giant aneurysms are found in 49% of these cases.

Two-Step Identification of N-, S-, R- and T-Cytoplasm Types in Onion Breeding Lines Using High-Resolution Melting (HRM)-Based Markers.

High-resolution melting (HRM) analysis is a powerful detection method for fast, high-throughput post-PCR analysis. A two-step HRM marker system was developed for identification of the N-, S-, R- and T-cytoplasms of onion. In the first step for the identification of N-, S- and R-cytoplasms, one forward primer was designed to the identical sequences of both and genes, and two reverse primers specific to the polymorphic sequences of and genes were used.

Bioinspired Propulsion System for a Thunniform Robotic Fish.

The paper describes a bioinspired propulsion system for a robotic fish model. The system is based on a combination of an elastic chord with a tail fin fixed on it. The tail fin is connected to a servomotor by two symmetric movable thrusts simulating muscle contractions.

Compensatory Effect of the Gene in Semi-Dwarf Spring Triticale (x Wittmack).

The dwarfness in many triticale cultivars is provided by the dominant () allele found in rye. However, along with conferring semi-dwarf phenotype to improve resistance to lodging, this gene also reduces grain size and weight and delays heading and flowering. () genes are plant-specific transcription factors that regulate plant growth, including stem growth, in terms of length and thickness, and leaf and fruit size.

[Possibilities of modern forms of antimicrobial peptides in the treatment of inflammatory pathology of the pharynx].

Inflammatory diseases of the pharynx occupy one of the leading places in the structure of otorhinolaryngological pathology. Acute and chronic inflammatory processes in the pharynx, as well as exacerbations of the latter, are faced by doctors of a wide range of specialties. Oropharyngeal swab laboratory testing is required to identify a specific pathogen, which takes time.

[The problem of chronic tonsillitis as a priority area of scientific research of the Department of Otorhinolaryngology named after acad. B.S. Preobrazhensky].

The article summarizes data on many years of versatile research on the problem of chronic tonsillitis, which were conducted at the Department of Otorhinolaryngology named after acad. B.S.

GALC variants affect galactosylceramidase enzymatic activity and risk of Parkinson's disease.

The association between glucocerebrosidase, encoded by GBA, and Parkinson's disease (PD) highlights the role of the lysosome in PD pathogenesis. Genome-wide association studies in PD have revealed multiple associated loci, including the GALC locus on chromosome 14. GALC encodes the lysosomal enzyme galactosylceramidase, which plays a pivotal role in the glycosphingolipid metabolism pathway.

Boiss. repeatome characterized using low-coverage NGS as a source of new FISH markers: Application in phylogenetic studies of the Triticeae.

. is polyploid grass species that grows in the eastern part of the Fertile Crescent, Afghanistan, and Middle Asia. It consists of tetraploid (4x) and hexaploid (6x) cytotypes (2 = 4x = 28, DD (Abdolmalaki et al.

Integrating Genetic and Chromosome Maps of : From Markers Visualization to Genome Assembly Verification.

The ability to directly look into genome sequences has opened great opportunities in plant breeding. Yet, the assembly of full-length chromosomes remains one of the most difficult problems in modern genomics. Genetic maps are commonly used in de novo genome assembly and are constructed on the basis of a statistical analysis of the number of recombinations.

Data-driven model discovery of ideal four-wave mixing in nonlinear fibre optics.

We show using numerical simulations that data driven discovery using sparse regression can be used to extract the governing differential equation model of ideal four-wave mixing in a nonlinear Schrödinger equation optical fibre system. Specifically, we consider the evolution of a strong single frequency pump interacting with two frequency detuned sidebands where the dynamics are governed by a reduced Hamiltonian system describing pump-sideband coupling. Based only on generated dynamical data from this system, sparse regression successfully recovers the underlying physical model, fully capturing the dynamical landscape on both sides of the system separatrix.

[Modern approaches to the surgical treatment of chronic tonsillitis].

At the moment, all over the world there are a large number of various methods of surgical treatment of chronic tonsillitis. In particular: classical tonsillectomy, cryosurgical method for removing the palatine tonsils, shaver tonsillectomy, monopolar and bipolar tonsillectomy, tonsillectomy with an ultrasonic scalpel, bipolar dissection using cutting instruments, laser tonsillectomy, coldablation. This article analyzes the existing methods, describes the fundamental technique for their implementation, as well as their advantages and disadvantages.

Antibacterial Activity of Medicinal Plants against Uropathogenic .

Urinary tract infections (UTIs) are one of the most common bacterial infections with uropathogenic (UPEC) being the most prevalent causative agent in both complicated and uncomplicated UTIs. Antibiotic resistance among UPEC has been already demonstrated against a wide variety of antibiotics and the situation is continuing to deteriorate increasing the rate of recurrence and the difficulty of treatment and prophylaxis. Recently, a big attention has been paid to non-antibiotic approaches as an alternative to conventional antibiotics.

Management of postoperative CSF rhinorrhea after retrosigmoid craniotomy: how I do it.

Background: Cerebrospinal fluid (CSF) rhinorrhea as a complication of retrosigmoid craniotomy does not occur often today. This complication is primarily associated with the petrous bone drilling during surgery.

Method: The management of this complication is shown by the example of the patient with a trigeminal schwannoma located in posterior cranial fossa operated through resrisigmoid craniotomy.