Analysing carbapenemases in hospital wastewater: Insights from intracellular and extracellular DNA using qPCR and digital PCR.

The widespread dissemination of carbapenem-resistant bacteria in wastewater systems, particularly from clinical sources, poses a significant public health risk. This study assessed the concentrations and distributions of extracellular DNA (exDNA) and intracellular DNA (iDNA) harboring carbapenemase genes in wastewater from six tertiary care hospitals in Germany. We collected a total of 36 samples, comprising six biological replicates from each hospital, and analysed them using quantitative real-time PCR (qPCR) and digital PCR (dPCR).

Potential Benefit of Probiotic E. Coli Nissle in Term Neonates.

Background:  Probiotics are often viewed as an immunity enhancing agent. The objective of this study was to investigate whether oral administration of Escherichia coli Nissle 1917 reduces the number of infections, their duration, and severity in the first 24 months after parturition in healthy neonates.

Subjects And Methods:  This prospective, confirmatory, randomised, double-blind, placebo-controlled study enrolled 567 healthy neonates from four German and two Polish sites.

PREVENTIVE EXAMINATIONS OF CHILDREN IN DIFFERENT COUNTRIES: SIMILARITIES AND DIFFERENCES.

Objective: The aim: The aim of this study was to compare, analize and establish differences the recommendations for well-child visits and screenings till the age of 5 years in different countries .

Patients And Methods: Materials and methods: The comparative analysis of the data from governmental recommendations for pediatricians from Germany, Ukraine and Poland is conducted. It was used the guideline for medical care for a child under 3 years (The Order №149) and for a healthy child from 4 to 18 years (The Order №434) in Ukraine, the book «Kinderuntersuchungsheft» in Germany and child's examination book «Książeczka zdrowia dziecka» in Poland.

Sex ratio of mirid populations shifts in response to hostplant co-infestation or altered cytokinin signaling .

Herbivore species sharing a host plant often compete. In this study, we show that host plant-mediated interaction between two insect herbivores - a generalist and a specialist - results in a sex ratio shift of the specialist's offspring. We studied demographic parameters of the specialist Tupiocoris notatus (Hemiptera: Miridae) when co-infesting the host plant Nicotiana attenuata (Solanaceae) with the generalist leafhopper Empoasca sp.

Neuropsychological variables and clinical status in anorexia nervosa: relationship between visuospatial memory and central coherence and eating disorder symptom severity.

Aim: To explore the relationship between visuospatial memory, weak central coherence, and eating disorder (ED) symptoms in anorexia nervosa (AN) inpatients.

Sample: 31 female AN inpatients.

Methods: Rey complex figure test (RCFT) was used to assess both visuospatial memory and central coherence.

Group cognitive remediation therapy for adult anorexia nervosa inpatients: first experiences.

Aim: In this study, we aim to explore the feasibility and acceptability of group cognitive remediation therapy (CRT) for anorexia nervosa (AN) inpatients, and to suggest modifications for future use.

Methods: We performed ten 45-min CRT sessions, once a week, in two consecutive groups of adult AN inpatients. The groups consisted of 14 and 20 participants, respectively.

Normative values of polysomnographic parameters in childhood and adolescence: quantitative sleep parameters.

Objective: To provide normative values for sleep macroarchitecture of healthy children aged 1-18 years using the AASM sleep scoring criteria, assessing the effects of gender, age, and Tanner pubertal stage.

Methods: One-night polysomnography was performed at subjects' habitual bedtimes in 16 laboratories on 209 healthy German children.

Results: Normal values of sleep macrostructure show significant age dependencies (p<0.

Swaddling: a traditional care method rediscovered.

Background: This study was undertaken to compare the sleep profiles of healthy infants in swaddling and sleeping bag conditions.

Methods: Polysomnographs of 85 healthy infants (40 in the study group, 45 in the control group) with a mean age of 7.5 weeks were recorded in the sleeping laboratory.

[Child abuse and violence as reasons for injuries in childhood: results of a monitoring of children and adolescents in South Brandenburg (Germany)].

Background: During childhood injuries are one of the most common reasons for a consultation, second only to infectious diseases. Not as rare as aspected these injuries are caused by maltreatment. The aim of this study is to show the frequency of non-accidental injuries (NAI) in the patient group from a pediatric hospital in East Germany.

A novel MECP2 mutation in a boy with neonatal encephalopathy and facial dysmorphism.

Methly-CpG-binding protein 2 (MECP2) mutations cause Rett syndrome in females. Here we report on a male infant with neonatal encephalopathy, myoclonic jerks, and irregular breathing patterns caused by a novel frameshift mutation in the MECP2 gene. In addition he has facial dysmorphisms previously not described in these patients.

[Unusual course of a Guillain-Barré syndrome in connection with an RS-Virus infection in a 10 months old infant].

Guillain Barré syndrome (GBS; Synonyma: Polyneuritis, Polyradikulitis) is an acute, inflammatory, demyelinating disease of the peripheral nerve system. Clinical hallmarks are symmetric muscle paralysis, areflexia and pronounced autonomic disturbances. Respiratory failure and cardiovascular instability are the main reasons for intensive care support in patients with GBS.

[Tracheal aplasia--an especially rare and dramatic anomaly].

Tracheal agenesis (TA), aplasia or total atresia of the trachea are congenital anomalies which are still incompatible with life. Despite the many attempts of different interventions, there are yet no promising, long-term methods of treatment. Only with sufficient proportion of the proximal or distal trachea available, it is possible to place a tracheostomy, which also opens up new vistas of life for the affected child.

[Aspiration of milk in healthy infant--cause of acute respiratory arrest?].

We present the case of 4month old female infant, who obviously suffered from a respiratory arrest during bottle-feed ing. After primary successful resuscitation the baby died after 49 days due to large intracranial hemorrages. The diagnosis of shaking trauma was based on the detailed medical examinations and on the case history taken from the police file.

[Multicystic renal tumor in a patient with WAGR syndrome].

The WAGR syndrome is a combination of Wilms' tumor, aniridia, genitourinary anomalies, and mental retardation. We report on a 2-year-old boy, who had a deletion of the aniridia gene PAX6 and the Wilms' tumor gene 1 (WT1 gene). At the age of 23 months, a 1.

Intrauterine torsion of a testicular teratoma: a case report.

Neonatal testicular tumors and intrauterine testicular torsions are very rare. The presented case is the first describing intrauterine torsion of a descended testis with a teratomatous tumor. Immediately after birth, right hemiscrotal swelling was seen in a preterm male newborn.

Obstructive sleep apnea syndrome in children: a state-of-the-art review.

Snoring and obstructive sleep apnea are a frequent problem not only in adults, but also in children and adolescents, as can be seen from current epidemiological data. The epidemiology, etiology, diagnosis, and management of obstructive sleep apnea syndrome (OSAS) in adults have been adequately established on the basis of evidential data. As a result of this, both physicians and the public are increasingly aware of OSAS in adults.

[Bilateral testicular masses in the scope of adrenogenital syndrome].

Testicular masses in male individuals with the adrenogenital syndrome (AGS) are a clinical and pathological diagnostic dilemma. The major differential diagnosis of gonadal nodules in this setting includes interstitial Leydig cell tumors and secondary benign tumors possibly of adrenal origin. We report a case of adrenogenital syndrome occurring in a 14-year-old boy.

[Reliability of home monitoring with event-recording compared with polysomnography in infants].

Aim Of The Study: Although reliable recognition of hypoxemia, apnea, bradycardia and tachycardia is absolutely necessary for home monitoring, many commercially available home monitors have not been sufficiently tested for their sensitivity. The purpose of this study was to determine the reliability of the home monitor VitaGuard 3000 by comparing it with the manual evaluation of full polysomnography (polysomnographic system Alice 3).

Patient And Methods: 20 infants (11 males; 9 females) aged between 5 and 40 weeks (12.

Longevity of Masimo and Nellcor pulse oximeter sensors in the care of infants.

Objective: Pulse oximetry is a standard of care for monitoring oxygenation in neonates. Associated with the use of pulse oximetry is the cost of patient sensors, especially if the sensor is designed for single-patient use. Pulse oximetry monitoring of sick newborns is routine and often lengthy and, if the pulse oximeter sensor is short-lived, can result in a significant portion in the cost of intensive care.

[Successful surfactant therapy of ARDS in an immunodepressed child].

The acute respiratory distress syndrome in childhood is a rare disease, but as in the past still plagued with a high mortality rate. It is caused by severe pneumoniaes or infectious diseases with multiorgan failure, aspiration, trauma or immunodepression. There are no therapeutic guidelines based on controlled studies.

[Nasal glioma as a rare cause of obstructed nasal breathing in a newborn infant].

Congenital midline nasal masses are rare entities occurring once in 20-40,000 births. They are often misdiagnosed or even missed. These tumors are most commonly present in newborn infants and children, but rarely they can be discovered in adults as well.

[Polysomnography in infancy--necessary or expensive luxury? Therapeutic and interventional consequences].

An increasing amount of polysomnographic studies are performed in early infancy. Complete pediatric sleep laboratory systems are commercially available and provide fast and exhaustive results if operated by trained personnel. Experience in evaluation is necessary, because a broad range of possible diagnostic findings exists, and artifacts are common.