Crystal, mol-ecular structure and Hirshheld surface analysis of 5-hy-droxy-3,6,7,8-tetra-meth-oxy-flavone.

The title compound (systematic name: 5-hydroxy-3,6,7,8-tetramethoxy-2-phenyl-4-chromen-4-one), CHO, is a flavone that was isolated from a butanol extract of the herb . The flavone mol-ecule is almost planar, with a dihedral angle between the planes of the benzo-pyran-4-one group and the attached phenyl ring of 6.4 (4)°.

Effect of plant flavonoids on the volume regulation of rat thymocytes under hypoosmotic stress.

Background: Cell volume regulation and volume-regulated anion channels are critical for cell survival in non-isosmotic conditions, and dysregulation of this system is detrimental. Although genes and proteins underlying this basic cellular machinery were recently identified, the pharmacology remains poorly explored.

Methods: We examined effects of 16 flavonoids on the regulatory volume decrease (RVD) of thymocytes under hypoosmotic stress assessed by light transmittance and on the activity of volume-sensitive chloride channel by patch-clamp technique.