Magnetic Resonance Imaging (MRI) and Hip Arthroscopy Correlations.

The hip is a complex joint which commonly generates referrals to orthoaedic surgeons. Hip arthroscopy continues to expand its indications for addressing hip pathology. Before operative intervention, the appropriate information must be obtained with magnetic resonance imaging playing a significant role in the workup given its outstanding characterization of soft tissue anatomy.

Tip-of-the-Iceberg Fractures: Small Fractures That Mean Big Trouble.

Objective: Several small and seemingly unimportant fractures are associated with other more serious injuries, usually to adjacent soft tissues. The purpose of this article is to discuss 11 of these injuries, in each case describing the fracture (the tip) and the injuries that lie beneath the surface (the iceberg).

Conclusion: Some fractures should be considered analogous to the tip of an iceberg.

Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.

We describe the development of a high performance liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues. Bulk lipids are extracted from biological samples by a modified Bligh/Dyer procedure in the presence of eight deuterated sterol standards to allow subsequent quantitation and determination of extraction efficiency. Sterols and other lipids are resolved by HPLC on a reverse-phase C18 column using a binary gradient of methanol and water, both containing 5mM ammonium acetate.