Theoretical-experimental studies of calmodulin-peptide interactions at different calcium equivalents.

We study the CaM-peptide interactions for four CaM-related peptides with different calcium equivalents, using the CaM-M124C- biosensor and Molecular Dynamics (MD). Due to the high sensitivity of the biosensor, we were able to calculate five based on the number of calcium equivalents for each peptide, showing a directly proportional relationship between the degree of calcium saturation and the increased affinity for the Calspermin, nNOS, and skMLSK peptides; while the CaV1.1 peptide has a degree of affinity independent of the number of calcium equivalent.