Publications by authors named "Erika L Knott"
Adrenocortical carcinoma (ACC) is a rare but deadly cancer for which few treatments exist. Here, we have undertaken a targeted bioinformatics study of The Cancer Genome Atlas (TCGA) ACC dataset focusing on the 30 genes encoding the γ-aminobutyric acid (GABA) system-an under-studied, evolutionarily-conserved system that is an emerging potential player in cancer progression. Our analysis identified a subset of ACC patients whose tumors expressed a distinct GABA system transcriptome.
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- Current methods for monitoring GABA in neural circuits are limited, lacking the ability to capture transient events.
- Researchers developed a new genetically encoded GABA sensor called iGABASnFR by applying techniques from an existing glutamate receptor sensor, which allows for real-time detection of GABA release in living organisms.
- iGABASnFR has been successfully used to investigate various GABA-related activities in zebrafish and mice, including tracking GABA levels in response to electric stimulation and monitoring changes during anesthesia or seizure events.
The potential application of mRNA for the identification of biological fluids using molecular techniques has been a recent development in forensic serology. Constitutively expressed housekeeping genes can assess the amount of mRNA recovered from a sample, establish its suitability for downstream applications, and provide a reference point to corroborate the identity of the fluid. qPCR was utilized to compare the expression levels of housekeeping genes from forensic-like body fluid stains to establish the most appropriate assessment of human mRNA quantity prior to profiling.
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