Gene expression profiles in mammary gland of male rats treated with genistein and methoxychlor.

Humans and wildlife are frequently exposed to mixtures of natural and synthetic endocrine-active compounds. To understand the impact of dietary phytoestrogen on the susceptibility to synthetic chemicals in the environment, we studied the effects of a binary mixture consisting of the isoflavone genistein and the pesticide methoxychlor on the development of the mammary gland. Sprague-Dawley rats were exposed to genistein at 800ppm, methoxychlor at 800ppm, or their combination through dietary administration to dams during pregnancy and lactation and to the offspring directly after weaning.

Modulation of mammary gland development in prepubertal male rats exposed to genistein and methoxychlor.

The estrogenic isoflavone genistein is a common dietary component that has been shown to affect reproductive development in experimental animals at high doses. The objective of the present study was to examine interactions of genistein and the hormonally active pesticide methoxychlor on mammary gland development in juvenile rats. Timed-pregnant Sprague-Dawley rats were fed a soy- and alfalfa-free diet containing different combinations of genistein (300 and 800 ppm) and methoxychlor (800 ppm).

Combined effects of dietary phytoestrogen and synthetic endocrine-active compound on reproductive development in Sprague-Dawley rats: genistein and methoxychlor.

Humans and wildlife are frequently exposed to mixtures of endocrine active-compounds (EAC). The objective of the present study was to investigate the potential of the phytoestrogen genistein to influence the reproductive developmental toxicity of the endocrine-active pesticide methoxychlor. Three levels of genistein (0, 300, or 800 ppm) and two levels of methoxychlor (0 or 800 ppm) were used in this study.