Aims: Xylene is most often employed in tissue processing protocols for paraffin embedding, but poses a health hazard. The aim of this study was to evaluate a solvent-free processing protocol that uses supercritical carbon dioxide (scCO(2) ) as an intermediate.
Methods And Results: A series of tests (with bovine tissues) was run, evaluating dehydration and tissue shrinkage in our new scCO(2) -based protocol as compared with routine processing using a graded ethanol and xylene series.