An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a methacrylate-based endodontic sealer.

The cytotoxicity of Epiphany root canal sealer at various concentrations from 25-800 microg/mL on human periodontal ligament (HPDL) fibroblasts was evaluated at 1, 3, and 7 days. Controls included untreated cells and cells treated with the vehicle for Epiphany suspension. Fibroblast viability was assessed by 2 methods, crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates.