Validation and method comparison of the use of densitometry to quantify monoclonal proteins in canine sera.

Background: Densitometric quantitation using serum protein electrophoresis (SPE) is used to monitor monoclonal proteins (M-proteins) in human patients but has not been validated in the dog. Serum globulin concentrations, species-specific radial immunodiffusion (RID), and ELISAs are currently used in veterinary medicine.

Objective: We aimed to compare four methods that quantify M-proteins using densitometry and biuret protein (dM-protein) measurements.