Using total adenosine triphosphate (tATP) measurements for cyanobacterial bloom monitoring and response assessment during algaecide treatments.

Total adenosine triphosphate (tATP) was investigated for its potential as a rapid indicator of cyanobacterial growth and algaecide effectiveness. tATP and other common bloom monitoring parameters were measured over the growth cycles of cyanobacteria and green algae in laboratory cultures and examined at a drinking water source during an active bloom. Strong correlations (R>0.

Critical Review on Bromate Formation during Ozonation and Control Options for Its Minimization.

Ozone is a commonly applied disinfectant and oxidant in drinking water and has more recently been implemented for enhanced municipal wastewater treatment for potable reuse and ecosystem protection. One drawback is the potential formation of bromate, a possible human carcinogen with a strict drinking water standard of 10 μg/L. The formation of bromate from bromide during ozonation is complex and involves reactions with both ozone and secondary oxidants formed from ozone decomposition, i.

Design and operational considerations in response to Legionella occurrence in Las Vegas Valley groundwater.

Legionella occurrence monitoring is not required by United States Environmental Protection Agency (USEPA) drinking water regulations, and few occurrence studies exist for Legionella in source water or distribution systems. Legionella occurrence was monitored in Las Vegas Valley (Las Vegas, Nevada, USA) drinking water sources, including non-treated surface water, seasonal groundwater (61 wells, before and after chlorination), finished water (after treatment at water treatment facilities), and chlorinated distribution system water (at 9 reservoirs and 75 sample locations throughout the network). Legionella pneumophila was detected at least once at each of the wells sampled before chlorination, with an overall positivity rate of 38% (343/908).

Benthic cyanobacteria: A utility-centred field study.

Although there is growing evidence that benthic cyanobacteria represent a significant source of toxins and taste and odour (T&O) compounds in water bodies globally, water utilities rarely monitor for them. Benthic cyanobacteria grow in an array of matrices such as sediments, biofilms, and floating mats, and they can detach and colonize treatment plants. The occurrence of compounds produced by benthic species across matrix and climate types has not been systematically investigated.

Ozone disinfection of waterborne pathogens and their surrogates: A critical review.

Viruses, Giardia cysts, and Cryptosporidium parvum oocysts are all major causes of waterborne diseases that can be uniquely challenging in terms of inactivation/removal during water and wastewater treatment and water reuse. Ozone is a strong disinfectant that has been both studied and utilized in water treatment for more than a century. Despite the wealth of data examining ozone disinfection, direct comparison of results from different studies is challenging due to the complexity of aqueous ozone chemistry and the variety of the applied approaches.

A critical review on operation and performance of source water control strategies for cyanobacterial blooms: Part II-mechanical and biological control methods.

This review summarizes current knowledge on mechanical (artificial mixing, hypolimnetic aeration, dredging, and sonication) and biological (biomanipulation, macrophytes, and straws) methods for the management of cyanobacterial blooms in drinking water sources. Emphasis has been given to (i) the mechanism of cyanobacterial control, (ii) successful and unsuccessful case studies, and (iii) factors influencing successful implementation. Most mechanical and biological control strategies offer long-term control.

A critical review on operation and performance of source water control strategies for cyanobacterial blooms: Part I-chemical control methods.

Cyanobacterial blooms produce nuisance metabolites (e.g., cyanotoxins and T&O compounds) thereby posing water quality management issues for aquatic sources used for potable water production, aquaculture, and recreation.

Comparative Assessment of Physical and Chemical Cyanobacteria Cell Lysis Methods for Total Microcystin-LR Analysis.

Standardization and validation of alternative cell lysis methods used for quantifying total cyanotoxins is needed to improve laboratory response time goals for total cyanotoxin analysis. In this study, five cell lysis methods (i.e.

Delayed Release of Intracellular Microcystin Following Partial Oxidation of Cultured and Naturally Occurring Cyanobacteria.

Oxidation processes can provide an effective barrier to eliminate cyanotoxins by damaging cyanobacteria cell membranes, releasing intracellular cyanotoxins, and subsequently oxidizing these toxins (now in extracellular form) based on published reaction kinetics. In this work, cyanobacteria cells from two natural blooms (from the United States and Canada) and a laboratory-cultured strain were treated with chlorine, monochloramine, chlorine dioxide, ozone, and potassium permanganate. The release of microcystin was measured immediately after oxidation (t ≤ 20 min), and following oxidant residual quenching (stagnation times = 96 or 168 h).

Using discrete and online ATP measurements to evaluate regrowth potential following ozonation and (non)biological drinking water treatment.

Water utilities must control microbial regrowth in the distribution system to protect public health. In this study, an adenosine triphosphate (ATP)-based biomass production potential test using indigenous bacterial communities were used to evaluate regrowth potential following ozonation with either biofiltration (BF) or sustained chlorination (SCl). Two full-scale water treatment plants with different upstream processes (i.

Using rapid quantification of adenosine triphosphate (ATP) as an indicator for early detection and treatment of cyanobacterial blooms.

Early detection of harmful cyanobacterial blooms allows identification of potential risk and appropriate selection of treatment techniques to prevent exposure in recreational water bodies and drinking water supplies. Here, luminescence-based adenosine triphosphate (ATP) analysis was applied to monitor and treat cultured and naturally occurring cyanobacteria cells. When evaluating lab-cultured Microcystis aeruginosa, ATP concentrations (≤252,000 pg/mL) had improved sensitivity and correlated well (R = 0.

Using upstream oxidants to minimize surface biofouling and improve hydraulic performance in GAC biofilters.

The combination of biological growth and particle loading can adversely affect hydraulic performance in drinking water biofilters. In this study, upstream oxidant addition was used to distribute biologically-derived filter clogging in granular activated carbon (GAC) biofilters. Oxidant penetration was assessed during pilot-scale operation and backwashing of dual media (GAC/sand) and multimedia (GAC/anthracite/sand) biofilters.

Investigation of biotransformation, sorption, and desorption of multiple chemical contaminants in pilot-scale drinking water biofilters.

The evolving demands of drinking water treatment necessitate processes capable of removing a diverse suite of contaminants. Biofiltration can employ biotransformation and sorption to remove various classes of chemicals from water. Here, pilot-scale virgin anthracite-sand and previously used biological activated carbon (BAC)-sand dual media filters were operated for ∼250 days to assess removals of 0.

Microbial community characterization of ozone-biofiltration systems in drinking water and potable reuse applications.

Microbial community structure in the ozone-biofiltration systems of two drinking water and two wastewater treatment facilities was characterized using 16S rRNA gene sequencing. Collectively, these datasets enabled comparisons by facility, water type (drinking water, wastewater), pre-oxidation (ozonation, chlorination), media type (anthracite, activated carbon), media depth, and backwash dynamics. Proteobacteria was the most abundant phylum in drinking water filters, whereas Bacteroidetes, Chloroflexi, Firmicutes, and Planctomycetes were differentially abundant in wastewater filters.

Varied influence of microcystin structural difference on ELISA cross-reactivity and chlorination efficiency of congener mixtures.

Enzyme-linked immunosorbent assay (ELISA) is an antibody-based analytical method that has been widely applied in water treatment utilities for the screening of toxic cyanobacteria metabolites such as microcystins (MCs). However, it is unknown how the minor structural difference of MCs may impact their chlorination kinetics and measurement via ELISA method. It was found in this study that, regardless of the experimental conditions (n = 21), there was no MC-YR or MC-LY residual, while different removal rates of other MCs were observed (MC-RR > MC-LR > MC-LA ∼ MC-LF) as measured by liquid chromatography tandem mass spectrometry (LC-MS/MS), which was consistent with the relative reactivity of the amino acid variables with free chlorine.

Ozone regeneration of granular activated carbon for trihalomethane control.

Spatial and temporal variations of trihalomethanes (THMs) in distribution systems have challenged water treatment facilities to comply with disinfection byproduct rules. In this study, granular activated carbon (GAC) and modified GAC (i.e.

Colonial cell disaggregation and intracellular microcystin release following chlorination of naturally occurring Microcystis.

Colonial cell disaggregation and release of intracellular microcystin were evaluated following chlorine treatment of naturally occurring Microcystis. Microscopic observations of water samples collected from Lake Mead, Nevada, USA, confirmed the presence of colonial Microcystis with cells protected by an outer sheath up to 30 μm thick. During chlorination, two stages of cell decomposition were observed, stage 1: colonial cell disaggregation, and stage 2: unicellular degradation.

Evaluating fluorescence spectroscopy as a tool to characterize cyanobacteria intracellular organic matter upon simulated release and oxidation in natural water.

Intracellular organic matter (IOM) from cyanobacteria may be released into natural waters following cell death in aquatic ecosystems and during oxidation processes in drinking water treatment plants. Fluorescence spectroscopy was evaluated to identify the presence of IOM from three cyanobacteria species during simulated release into natural water and following oxidation processes (i.e.

Effect of oxidant exposure on the release of intracellular microcystin, MIB, and geosmin from three cyanobacteria species.

The release of intracellular microcystin-LR (MC-LR), 2-methylisoborneol (MIB), and geosmin was investigated after the oxidation of three cyanobacteria (Microcystis aeruginosa (MA), Oscillatoria sp. (OSC), and Lyngbya sp. (LYN)).

Using digital flow cytometry to assess the degradation of three cyanobacteria species after oxidation processes.

Depending on drinking water treatment conditions, oxidation processes may result in the degradation of cyanobacteria cells causing the release of toxic metabolites (microcystin), odorous metabolites (MIB, geosmin), or disinfection byproduct precursors. In this study, a digital flow cytometer (FlowCAM(®)) in combination with chlorophyll-a analysis was used to evaluate the ability of ozone, chlorine, chlorine dioxide, and chloramine to damage or lyse cyanobacteria cells added to Colorado River water. Microcystis aeruginosa (MA), Oscillatoria sp.

Intracellular organic matter from cyanobacteria as a precursor for carbonaceous and nitrogenous disinfection byproducts.

The formation of total organic halogen (TOX), carbonaceous disinfection byproducts (DBPs) (trihalomethanes (THMs) and haloacetic acids (HAAs)), and nitrogenous DBPs (trichloronitromethane (TCNM) or chloropicrin, haloacetonitriles (HANs), and nitrosamines) was examined during the chlorination or chloramination of intracellular organic matter (IOM) extracted from Microcystis aeruginosa, Oscillatoria sp. (OSC), and Lyngbya sp. (LYN).

Prediction of micropollutant elimination during ozonation of municipal wastewater effluents: use of kinetic and water specific information.

Ozonation is effective in improving the quality of municipal wastewater effluents by eliminating organic micropollutants. Nevertheless, ozone process design is still limited by (i) the large number of structurally diverse micropollutants and (ii) the varying quality of wastewater matrices (especially dissolved organic matter). These issues were addressed by grouping 16 micropollutants according to their ozone and hydroxyl radical ((•)OH) rate constants and normalizing the applied ozone dose to the dissolved organic carbon concentration (i.

Development of surrogate correlation models to predict trace organic contaminant oxidation and microbial inactivation during ozonation.

The performance of ozonation in wastewater depends on water quality and the ability to form hydroxyl radicals (·OH) to meet disinfection or contaminant transformation objectives. Since there are no on-line methods to assess ozone and ·OH exposure in wastewater, many agencies are now embracing indicator frameworks and surrogate monitoring for regulatory compliance. Two of the most promising surrogate parameters for ozone-based treatment of secondary and tertiary wastewater effluents are differential UV(254) absorbance (ΔUV(254)) and total fluorescence (ΔTF).

Evaluation of enhanced coagulation pretreatment to improve ozone oxidation efficiency in wastewater.

Enhanced coagulation (EC) using ferric chloride was evaluated as a pretreatment process to improve the efficiency of ozone (O3) for the oxidation of trace organic contaminants in wastewater. At the applied dosages (10-30 mg/L as Fe), EC pretreatment removed between 10 and 47% of the dissolved organic carbon (DOC) from the three wastewaters studied. Size exclusion chromatography (SEC) showed that EC preferentially removed higher apparent molecular weight (AMW) compounds.

Evaluation of UV/H(2)O(2) treatment for the oxidation of pharmaceuticals in wastewater.

Advanced oxidation treatment using low pressure UV light coupled with hydrogen peroxide (UV/H(2)O(2)) was evaluated for the oxidation of six pharmaceuticals in three wastewater effluents. The removal of these six pharmaceuticals (meprobamate, carbamazepine, dilantin, atenolol, primidone and trimethoprim) varied between no observed removal and >90%. The role of the water quality (i.