Enhancing cyclotide bioproduction: harnessing biological synthesis methods and various expression systems for large-scale manufacturing.

Peptide-based medications hold immense potential in addressing a wide range of human disorders and discomforts. However, their widespread utilization encounters two major challenges: preservation and production efficiency. Cyclotides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs), exhibit unique characteristics, such as a cyclic backbone and cystine knot, enhancing their stability and contributing to a wide range of pharmacological properties exhibited by these compounds.

Incidents in the context of pre-hospital care by ambulances: contributions to patient safety.

Objectives: to analyze the occurrence of incidents in the context of mobile terrestrial pre-hospital care.

Methods: a descriptive research was carried out through the observation of 239 treatments performed by 22 healthcare professionals at the Mobile Emergency Care Service, located in Baixada Fluminense, Rio de Janeiro, Brazil. Fisher's exact test and chi-square test were used for data analysis.

Transcriptional Profiling of SARS-CoV-2-Infected Calu-3 Cells Reveals Immune-Related Signaling Pathways.

The COVID-19 disease, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), emerged in late 2019 and rapidly spread worldwide, becoming a pandemic that infected millions of people and caused significant deaths. COVID-19 continues to be a major threat, and there is a need to deepen our understanding of the virus and its mechanisms of infection. To study the cellular responses to SARS-CoV-2 infection, we performed an RNA sequencing of infected vs.

enhances tomato drought tolerance by improving photosynthesis, nutrient uptake and enzymatic antioxidant response.

Functional symbiosis with fungal endophytes can help plants adapt to environmental stress. is one of the most abundant fungal taxa associated with roots of subsp. , a grass growing in sea cliffs.

ABO blood group and link to COVID-19: A comprehensive review of the reported associations and their possible underlying mechanisms.

ABO blood group is long known to be an influencing factor for the susceptibility to infectious diseases, and many studies have been describing associations between ABO blood types and COVID-19 infection and severity, with conflicting findings. This narrative review aims to summarize the literature regarding associations between the ABO blood group and COVID-19. Blood type O is mostly associated with lower rates of SARS-CoV-2 infection, while blood type A is frequently described as a risk factor.

A Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass.

Some microbiome components can provide functions that extend the capabilities of plants, increasing the environmental adaptability and performance of holobionts. subsp. is a perennial grass adapted to rocky sea cliffs, where soil and nutrients are very limited, and exposure to salinity is continuous.

The Role of Fungal Microbiome Components on the Adaptation to Salinity of subsp. .

subsp. is a perennial grass that inhabits sea cliffs, a habitat where salinity and low nutrient availability occur. These plants have a rich fungal microbiome, and particularly common are their associations with in aboveground tissues and with and in roots.

Pre-hospital assistance by ambulance in the context of coronavirus infections.

Objective: To reflect on the safe care exercised by the pre-hospital care team by emergency ambulance in times of coronavirus infection.

Method: A reflection and description of how to provide safe care to the patient and the professional during pre-hospital care in times of coronavirus infection.

Results: To ensure the health of all those involved in the care, health professionals who work in pre-hospital care by emergency ambulance should use the recommended Personal Protective Equipment (PPE), such as the use of surgical masks and N95, N99, N100, PFF2 or PFF3, the use of an apron or overall, goggles and face shield, gloves and a hat.

Communication between pre-hospital and intra-hospital emergency medical services: literature review.

Objectives: to analyze, according to the scientific literature, communication strategies in the transfer of cases between pre-hospital and in-hospital services and their contributions to patient safety.

Methods: this is a literature review study, that is, one that aims to gather and synthesize research results on the subject in a systematic and orderly manner.

Results: ten articles were published, published between 2010 and 2018, and two points of discussion emerged: use of mnemonics; and barriers to transferring a case.

Conservation paradox: Large-scale mining waste in protected areas in two global hotspots, southeastern Brazil.

Irreversible losses of natural areas and damage to ecosystem services are caused by large-scale mining. These effects are prominent in Minas Gerais, which is home to a mining industry with hundreds of tailings dams concentrated in global hotspots: the Cerrado and the Atlantic Forest. We present an overview of the spatial overlap between protected areas (PAs), priority conservation areas (PCAs), tailings dams, and their influence areas.

A Survey of Culturable Fungal Endophytes From subsp. , a Grass From Marine Cliffs, Reveals a Core Microbiome.

subsp. is a perennial grass that inhabits sea cliffs of the Atlantic coasts of Europe. In this unhospitable environment plants grow in rock crevices and are exposed to abiotic stress factors such as low nutrient availability, wind, and salinity.

Soil DNA pyrosequencing and fruitbody surveys reveal contrasting diversity for various fungal ecological guilds in chestnut orchards.

Fungal diversity in Mediterranean forest soils is poorly documented, particularly when considering saprobic and pathogenic organisms. Next-generation sequencing (NGS) methods applied to soil fungi provide the opportunity to unveil the most inconspicuous functional guilds (e.g.

Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS.

Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection.

A new effective assay to detect antimicrobial activity of filamentous fungi.

The search for new antimicrobial compounds and the optimization of production methods turn the use of antimicrobial susceptibility tests a routine. The most frequently used methods are based on agar diffusion assays or on dilution in agar or broth. For filamentous fungi, the most common antimicrobial activity detection methods comprise the co-culture of two filamentous fungal strains or the use of fungal extracts to test against single-cell microorganisms.

Effect of competitive interactions between ectomycorrhizal and saprotrophic fungi on Castanea sativa performance.

In Northeast of Portugal, the macrofungal community associated to chestnut tree (Castanea sativa Mill.) is rich and diversified. Among fungal species, the ectomycorrhizal Pisolithus tinctorius and the saprotroph Hypholoma fasciculare are common in this habitat.