Publications by authors named "Eric Paget"
Article Synopsis
- Using oral swabs to collect remnants of stomach content during rumination in dairy cows can closely replicate ruminal bacterial communities, making it beneficial for large-scale studies on the rumen microbiome.
- The quality of these swabs can vary, affecting their ability to accurately reflect the microbiome due to factors such as sampling time, cow behavior, and swab color.
- Our research shows that darker swabs correlate with increased bacterial diversity while lighter swabs display greater variation; darker swabs also relate to the ruminal fungal community, indicating their potential as a more accurate representation of the ruminal environment.
The search for safe and efficient new antifungal compounds for agriculture has led to more efforts in finding new modes of action. This involves the discovery of new molecular targets, including coding and non-coding RNA. Rarely found in plants and animals but present in fungi, group I introns are of interest as their complex tertiary structure may allow selective targeting using small molecules.
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- The order of rust fungi includes over 7,000 species that significantly affect agriculture, horticulture, forestry, and ecosystems.
- Phakopsora pachyrhizi, the fungus responsible for Asian soybean rust disease, is a prime example of this impact and has a complex genome that has been challenging to assemble accurately.
- Researchers sequenced three genomes of P. pachyrhizi, revealing a size of up to 1.25 Gb and a high transposable element content (~93%), demonstrating the role of these elements in host adaptation, stress responses, and genetic variability.
Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biological activity of many therapeutic proteins. We have investigated the capacity of tobacco (Nicotiana tabacum) chloroplasts to efficiently form disulphide bonds in proteins by expressing in this plant cell organelle a well-known bacterial enzyme, alkaline phosphatase, whose activity and stability strictly depend on the correct formation of two intramolecular disulphide bonds.
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