Simultaneous high-temperature gas chromatography with flame ionization and mass spectrometric analysis of monocarboxylic acids and acylglycerols in biofuels and biofuel intermediate products.

Triacyl-, diacyl- and monoacylglycerols (TAGs, DAGs, MAGs) along with monocarboxylic acids (MCAs) are intermediate products in many triacylglycerol oil-to-biofuel conversion pathways. Accumulation of these compounds leads to poor biofuel characteristics and may result in fuel system damage. We developed a method for simultaneous identification and quantification of a wide range of MCAs (C-C), MAGs, DAGs, and TAGs.