Wood-Ljungdahl pathway encoding anaerobes facilitate low-cost primary production in hypersaline sediments at Great Salt Lake, Utah.

Little is known of primary production in dark hypersaline ecosystems despite the prevalence of such environments on Earth today and throughout its geologic history. Here, we generated and analyzed metagenome-assembled genomes (MAGs) organized as operational taxonomic units (OTUs) from three depth intervals along a 30-cm sediment core from the north arm of Great Salt Lake, Utah. The sediments and associated porewaters were saturated with NaCl, exhibited redox gradients with depth, and harbored nitrogen-depleted organic carbon.

Fluid-driven aseismic fault slip with permeability enhancement and dilatancy.

Injection-induced seismicity and aseismic slip often involve the reactivation of long-dormant faults, which may have extremely low permeability prior to slip. In contrast, most previous models of fluid-driven aseismic slip have assumed linear pressure diffusion in a fault zone of constant permeability and porosity. Slip occurs within a frictional shear crack whose edge can either lag or lead pressure diffusion, depending on the dimensionless stress-injection parameter that quantifies the prestress and injection conditions.

Iron Minerals Influence the Assembly of Microbial Communities in a Basaltic Glacial Catchment.

The influence of mineralogy on the assembly of microbial communities in glacial environments has been difficult to assess due to complications in isolating mineralogy from other variables. Here we assess the abundance and composition of microbial communities that colonized defined minerals incubated for 12 months in two meltwater streams (N and S) emanating from Kaldalónsjökull (Kal), a basalt-hosted glacier in Iceland. The two streams shared similar meltwater geochemistry as well as bedrock and proglacial sediment elemental compositions.

Hindcasting injection-induced aseismic slip and microseismicity at the Cooper Basin Enhanced Geothermal Systems Project.

There is a growing recognition that subsurface fluid injection can produce not only earthquakes, but also aseismic slip on faults. A major challenge in understanding interactions between injection-related aseismic and seismic slip on faults is identifying aseismic slip on the field scale, given that most monitored fields are only equipped with seismic arrays. We present a modeling workflow for evaluating the possibility of aseismic slip, given observational constraints on the spatial-temporal distribution of microseismicity, injection rate, and wellhead pressure.

Lithogenic hydrogen supports microbial primary production in subglacial and proglacial environments.

Life in environments devoid of photosynthesis, such as on early Earth or in contemporary dark subsurface ecosystems, is supported by chemical energy. How, when, and where chemical nutrients released from the geosphere fuel chemosynthetic biospheres is fundamental to understanding the distribution and diversity of life, both today and in the geologic past. Hydrogen (H) is a potent reductant that can be generated when water interacts with reactive components of mineral surfaces such as silicate radicals and ferrous iron.

Fault valving and pore pressure evolution in simulations of earthquake sequences and aseismic slip.

Fault-zone fluids control effective normal stress and fault strength. While most earthquake models assume a fixed pore fluid pressure distribution, geologists have documented fault valving behavior, that is, cyclic changes in pressure and unsteady fluid migration along faults. Here we quantify fault valving through 2-D antiplane shear simulations of earthquake sequences on a strike-slip fault with rate-and-state friction, upward Darcy flow along a permeable fault zone, and permeability evolution.

Geologic legacy spanning >90 years explains unique Yellowstone hot spring geochemistry and biodiversity.

Little is known about how the geological history of an environment shapes its physical and chemical properties and how these, in turn, influence the assembly of communities. Evening primrose (EP), a moderately acidic hot spring (pH 5.6, 77.

Origin and Evolution of Flavin-Based Electron Bifurcating Enzymes.

Twelve evolutionarily unrelated oxidoreductases form enzyme complexes that catalyze the simultaneous coupling of exergonic and endergonic oxidation-reduction reactions to circumvent thermodynamic barriers and minimize free energy loss in a process known as flavin-based electron bifurcation. Common to these 12 bifurcating (Bf) enzymes are protein-bound flavin, the proposed site of bifurcation, and the electron carrier ferredoxin. Despite the documented role of Bf enzymes in balancing the redox state of intracellular electron carriers and in improving the efficiency of cellular metabolism, a comprehensive description of the diversity and evolutionary history of Bf enzymes is lacking.

A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle.

Background: The 2014-2016 Ebola virus (EBOV) outbreak in West Africa highlighted the need for improved therapeutic options against this virus. Approaches targeting host factors/pathways essential for the virus are advantageous because they can potentially target a wide range of viruses, including newly emerging ones and because the development of resistance is less likely than when targeting the virus directly. However, systematic approaches for screening host factors important for EBOV have been hampered by the necessity to work with this virus at biosafety level 4 (BSL4).

Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses.

Infection with Junín virus (JUNV) is currently being effectively managed in the endemic region using a combination of targeted vaccination and plasma therapy. However, the long-term sustainability of plasma therapy is unclear and similar resources are not available for other New World arenaviruses. As a result, there has been renewed interest regarding the potential of drug-based therapies.

Ebola virus VP24 interacts with NP to facilitate nucleocapsid assembly and genome packaging.

Ebola virus causes devastating hemorrhagic fever outbreaks for which no approved therapeutic exists. The viral nucleocapsid, which is minimally composed of the proteins NP, VP35, and VP24, represents an attractive target for drug development; however, the molecular determinants that govern the interactions and functions of these three proteins are still unknown. Through a series of mutational analyses, in combination with biochemical and bioinformatics approaches, we identified a region on VP24 that was critical for its interaction with NP.

Assessing the contribution of interferon antagonism to the virulence of West African Ebola viruses.

The current Ebola virus (EBOV) outbreak in West Africa is unprecedented in terms of both its size and duration, and there has been speculation and concern regarding the potential for EBOV to increase in virulence as a result of its prolonged circulation in humans. Here we investigate the relative potency of the interferon (IFN) inhibitors encoded by EBOVs from West Africa, since an important EBOV virulence factor is inhibition of the antiviral IFN response. Based on this work we show that, in terms of IFN antagonism, the West African viruses display no discernible differences from the prototype Mayinga isolate, which corroborates epidemiological data suggesting these viruses show no increased virulence compared with those from previous outbreaks.

A supershear transition mechanism for cracks.

Seismic data indicate that fault ruptures follow complicated paths with variable velocity because of inhomogeneities in initial stress or fracture energy. We report a phenomenon unique to three-dimensional cracks: Locally stronger fault sections, rather than slowing ruptures, drive them forward at velocities exceeding the shear wave speed. This supershear mechanism differentiates barrier and asperity models of fault heterogeneity, which previously have been regarded as indistinguishable.