One of the most curious findings associated with the discovery of mimivirus (APMV) was the presence of many proteins and RNAs within the virion. Although some hypotheses on their role in Acanthamoeba infection have been put forward, none have been validated. In this study, we directly transfected mimivirus DNA with or without additional proteinase K treatment to extracted DNA into In this way, it was possible to generate infectious APMV virions, but only without extra proteinase K treatment of extracted DNA.
View Article and Find Full Text PDFHuman plasma paraoxonase (HuPON1) is a high density lipoprotein (HDL)-bound enzyme exhibiting antiatherogenic properties. The molecular basis for the binding specificity of HuPON1 to HDL has not been established. Isolation of HuPON1 from HDL requires the use of detergents.
View Article and Find Full Text PDF