Lipidomics differentiation of soft-shelled turtle strains using hydrophilic interaction liquid chromatography and mass spectrometry.

Hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) was used as an accurate and sensitivity technique to separate and characterize the phospholipids in three strains of soft-shelled turtles. Under the optimized experimental parameters, the lipidomic profiles were successfully acquired, and a total of 55 phospholipid molecular species were identified. The principal component analysis (PCA) was applied to normalize the relative amounts of the phospholipid molecular species and revealed the differences among three strains of soft-shelled turtle, including Qingxi Huabie (QXH, GS-01-003-2008), Zhexin Wubie (ZXW, GS-02-005-2015), and Zhewu Erhao (ZWE, unregistered).

[Effects of naokang erhao decoction on cognitive ability and hippocampal apoptosis-related proteins in diabetic rats].

Objective: To investigate the effects of Naokang Erhao decoction on the cognitive ability and the expression of Caspase-3, Bax and Bcl-2 proteins in hippocampus of type 2 diabetic rats.

Method: The diabetes mellitus (DM) rat model was produced by intraperitoneal injection of streptozotocin and fed with high fat and sucrose diet. The Naokang Erhao-treated rats were intragastrically given different doses of Naokang Erhao, whereas the control and DM model groups were given double distilled water for 4 consecutive weeks.