The Low Genetic Diversity of Dientamoeba fragilis Isolates in Southwest Turkey and Analysis of Clinical Findings.

Purpose: Dientamoeba fragilis (D. fragilis) is a common intestinal protozoan with a global distribution. In the present study, we aimed to determine genetic diversity of D.

: A Mysterious Member of the Gut Microbiome.

is the most common gastrointestinal protist found in humans and animals. Although the clinical significance of remains unclear, the organism is increasingly being viewed as a commensal member of the gut microbiome. However, its impact on the microbiome is still being debated.

Subtype Distribution of in Türkiye.

is an anaerobic protozoan with global importance because of infecting a variety of hosts and having high prevalence in many countries. isolates display remarkable genetic differences, and many subtypes (STs) have currently been defined based on polymorphism in coding gene. Each 25 subtype may have different characteristics such as pathogenicity, host specificity, and structural variations.

Wound Myiasis in Diabetic Foot Ulcer: Calliphoridae and Sarcophagidae Family.

Background: Myiasis is a parasitic infestation of tissues or body cavities of mammals with dipterous larvae. The patients with diabetic foot ulcers are more vulnerable to acquiring infestation; however, the infestation may be neglected and mistreated in some cases.

Methods: Data were collected of twelve myiasis cases with diabetic foot ulcers in Nazli-Selim Eren Chronic Wound and Infections Care Unit, Aydin, Turkey between 2017 and 2019.

Investigation of the relationship between obesity and Blastocystis infection in an adult population in Aydin, Turkey.

Blastocystis is one of the most frequent protozoa in human faecal samples, however, little is known about its relation with obesity. The present study aimed to analyse Blastocystis infection and subtypes in three adult populations classified according to body mass index (BMI). Faecal samples from 346 individuals were classified according to BMI: control (124 cases), overweight (110 cases), and obese (112 cases).

Investigation of Frequency in Faecal Samples of Patients with Infection by Polymerase Chain Reaction.

Objective: () is a flagellated protozoan with an amoeba-like morphology, located in the gastrointestinal tract. The hypothesis was that the parasite was transported by () eggs. This study aimed to determine the association of and with the genotypes of the identified strain of .

First Molecular Characterisation of from Experimental Rats in Turkey and Comparison of the Frequencies Between Obese and Non-obese Groups.

Objective: is a zoonotic protozoan that infects a wide range of animals, including humans and rodents. This study aimed to determine the frequency and subtype distribution of in laboratory rats at a laboratory animal facility in Turkey.

Methods: This study included 54 male Sprague-Dawley rats from Aydın Adnan Menderes University Laboratory Animal Center.

Microsatellite-Based Genotyping, Analysis of Population Structure, Presence of Virus (TVV) and Isolates from Southwest of Turkey.

Background: The present study aimed to determine genetic diversity of isolates with microsatellite markers in Turkey (Nov 2015 to 2016) and to create a web-based microsatellite typing (MT) approach for the global interpretation of the data. In addition, the endosymbiosis of and virus (TVV) in the isolates was also examined.

Methods: The allele sizes for each locus were calculated and microsatellite types were determined according to the allele profiles.

Subtype Distribution of in Pregnant Women and Analysis of Possible Risk Factors.

Objective: Since the identification of subtypes (ST) in the last decade, much has been learned about the genetic diversity of isolates in different populations, except pregnant women. The objective of this study is to investigate the genetic diversity of in pregnant women and analyse some demographic factors.

Methods: The faecal samples from 100 pregnant women were collected at an Obstetrics and Gynecology Department in Muğla, Turkey.

Molecular characterisation of Trichomonas vaginalis isolates in Southwest Turkey with multilocus sequence typing and genetic structure analysis in relation to different countries.

Trichomonas vaginalis, a flagellated protozoan parasite, is among the most common sexually transmitted pathogens in the world. The present study aimed to identify the genetic profiles of T. vaginalis in the southwest of Turkey with multilocus sequence typing (MLST) and to analyse the genetic structure of the parasite in a collection of isolates from different countries.

The Retrospective Analysis of spp. Results in Aydın Adnan Menderes University Faculty of Medicine Hospital Parasitology Laboratory.

Objective: spp. is one of the most common ectoparasites in humans. The aim of the present study is to evaluate the positivity of spp.

Retrospective Analysis of Cystic Echinococcosis Results in Aydın Adnan Menderes University Training and Research Hospital Parasitology Laboratory Between 2005 and 2017.

Objective: is the causative helminth of cystic echinococcosis (CE). The parasite is known to form fluid-filled cysts that grow slowly in the internal organs, particularly the liver and/or lungs. This disease is still important in terms of public health and economically in Turkey and other countries where animal husbandry is widespread.

[Subtype Distribution of Blastocystis spp. with DNA Barcoding and Evaluation of Diagnostic Methods].

Blastocystis spp. is one of the most common protozoa in Turkey and throughout the world; laboratory diagnosis, genetic diversity and clinical features are among the most controversial topics related to the parasite. The aims of the present study were to investigate the subtype distribution of Blastocystis spp.

[Transfection of Leishmania tropica with green fluorescent protein (gfp) gene and investigation of the in vitro drug effect].

Cutaneous leishmaniasis (CL) is a parasitic disease transmitted by vector sand flies Phlebotomus and Lutzomyia. This disease is characterized by long time non-healing skin lesions, and caused by Leishmania species. CL is the most common infection in Eastern and Southeastern Anatolia in Turkey and L.

Retrospective Analysis of Serology Results from Adnan Menderes University Faculty of Medicine Parasitology Laboratory from 2007 to 2017.

Objective: is a common apicomplexan parasite of humans and can cause significant morbidity and mortality due to congenital transmission and in patients with immune deficiency. The aim of this study was to evaluate serology results of 11 years and to determine compatibility of serologic diagnosis methods.

Methods: The study was conducted between 2007 and 2017, and anti- IgG antibodies were investigated by an in-house Enzyme Linked Immunosorbent Assay (ELISA) and Indirect Fluorescence Antibody (IFA) methods.

Investigation of the Applicability of a Rapid Diagnosis Test in the Diagnosis of Cystic Echinococcosis.

Objective: Echinococcus granulosus, the etiological agent of cystic echinococcosis (CE) in humans and livestock, is a widely distributed zoonotic pathogen tapeworm. The infection is transmitted to humans by the ingestion of E. granulosus eggs released in the feces of definitive hosts such as dogs.

[Genotyping of Echinococcus granulosus isolates by sequencing of mitochondrial cytochrome C oxidase subunit 1 (cox1) gene in Aydin].

Cyst hydatid (CH) is a zoonotic infection that is characterized by the development of metacestode form of Echinococcus granulosus primarily in liver of humans and ruminants. With a worldwide distribution, the infection is still considered as an important parasitic disease that threatens the public health in Turkey as in the other developing countries. Morphological and biological features of parasite fail to discriminate isolates for typing so molecular methods should be used for this purpose.

Use of Internal Transcribed Spacer Sequence Polymorphisms as a Method for Trichomonas vaginalis Genotyping.

Objective: Trichomonas vaginalis is the most common non-viral, sexually transmitted pathogen with a worldwide distribution. The aim of the present study was to design a new genotyping tool for T. vaginalis isolates using internal transcribed spacer (ITS) sequences.

Determination of Trichomonas vaginalis Genotypes Using PCR-Restriction Fragment Length Polymorphism (RFLP).

Objective: Trichomonas vaginalis is an anaerobic protozoon and the most common non-viral sexually transmitted pathogen. The present study was designed to determine the genotypes of T. vaginalis using polymerase chain reaction (PCR)-restriction fragment length polymorphism of actin gene.

The Distribution of Subtypes among School-aged Children in Mugla, Turkey.

Background: is a common protozoon that inhabits human intestinal tract and has a worldwide distribution. This study aimed to determine subtype (ST) distribution of among school-aged children in a western city of Turkey between Mar and Jun 2014.

Methods: This cross-sectional study was conducted among primary school children in Mugla between Mar and Jun 2014.

[Investigation of in vitro metronidazole resistance in the clinical isolates of Trichomonas vaginalis].

Trichomonas vaginalis, a flagellated, urogenital anaerobic protozoon is reported as an important cause of vaginitis with a global distribution. Although metronidazole is the primary choice of drug for the treatment of trichomoniasis, the presence of resistant isolates from many different countries highlights the need of novel drugs for the treatment. Many studies from Turkey mostly dealing with the in vitro effects of compounds and natural products against T.

Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings.

Objective: To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases.

Methods: Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct microscopy and cultivated in Jones medium. Blastocystis positive cultures were subjected to DNA isolation and subtypes were identified by sequencing of barcode region.

Multilocus sequence typing of Blastocystis isolates in Aydin, Turkey.

Blastocystis, a stramenopile protozoon of the human gastrointestinal tract, has a worldwide distribution. Multilocus sequence typing (MLST) is described as a portable, universal, and definitive method for accurate strain identification of microorganisms and was recently used for detecting intra-subtype variability of Blastocystis. The present study aimed to determine MLST sequence types in Blastocystis isolates from a routine diagnostic laboratory at Adnan Menderes University, Training and Research Hospital, Turkey.

[Genotyping of Giardia intestinalis strains isolated from humans in Aydin, Turkey].

Giardia intestinalis which is a flagellate, intestinal protozoon of humans and a variety of mammalian species, shows worldwide distribution. To date, eight genotypes of the parasite have been identified. Among these genotypes, assemblage A and B have zoonotic characteristics with low host specificity, thus they are responsible for the human infections.

Blastocystis subtypes in cancer patients: Analysis of possible risk factors and clinical characteristics.

The aim of the present study was to examine the frequency and genetic diversity of Blastocystis in cancer patients of a Medical Oncology Department in Aydın, Turkey. Patients' stool samples were examined between January 2013 and February 2014 by both microscopy and culture methods. Culture positive samples were subjected to DNA isolation and Sequence Tagged Site (STS)-PCR analysis.