A Monochromatically Excitable Green-Red Dual-Fluorophore Fusion Incorporating a New Large Stokes Shift Fluorescent Protein.

Genetically encoded sensors enable quantitative imaging of analytes in live cells. Sensors are commonly constructed by combining ligand-binding domains with one or more sensitized fluorescent protein (FP) domains. Sensors based on a single FP can be susceptible to artifacts caused by changes in sensor levels or distribution in vivo.

Affinity Purification of GO-Matryoshka Biosensors from for Quantitative Ratiometric Fluorescence Analyses.

Genetically encoded biosensors are powerful tools for quantitative visualization of ions and metabolites . Design and optimization of such biosensors typically require analyses of large numbers of variants. Sensor properties determined such as substrate specificity, affinity, response range, dynamic range, and signal-to-noise ratio are important for evaluating data.