Immune and physiological responses in to ammonia-N stress: a multi-omics approach.

Ammonia-N stress is a significant environmental factor that adversely affects the health and productivity of aquaculture species. This study investigates the effects of ammonia-N stress on the shrimp through a combination of biochemical, histological, transcriptomic, and metabolomic analyses. Shrimp were exposed to ammonia-N stress for 12 and 96 hours, and key markers of oxidative stress, nitrogen metabolism, immune response, and overall health were assessed.

Interactions of a PFOS/sodium nitrite mixture in Chinese mitten crab (Eriocheir sinensis): Impacts on survival, growth, behavior, energy metabolism and hepatopancreas transcriptome.

Perfluorooctanesulfonic acid (PFOS) and sodium nitrite may have complex adverse effects on aquatic animals. This study assessed the interactive effects of PFOS and sodium nitrite on Chinese mitten crab (Eriocheir sinensis). A 2 × 3 factorial experiment with 0, 0.

Combined exposure effects: Multilevel impact analysis of cycloxaprid and microplastics on Penaeus vannamei.

In real environments, multiple pollutants often coexist, so studying the impact of a single pollutant does not fully reflect the actual situation. Cycloxaprid, a new neonicotinoid pesticide, poses significant ecological risks due to its unique mechanism and widespread distribution in aquatic environments. Additionally, the ecological effects of microplastics, another common environmental pollutant, cannot be overlooked.

Effects of dietary methionine on the growth and protein synthesis of juvenile Chinese mitten crabs () fed fish meal-free diets.

This study investigated the effects of dietary methionine (Met) on growth performance and protein synthesis in juvenile Chinese mitten crabs () fed fish meal (FM)-free diets. Three diets free of FM containing 0.48% (LM), 1.

Molecular Insights into the CO Mineralization Process with Tricalcium Silicate.

CO mineralization, a pivotal technology in CO capture, storage, and utilization, promises to convert industrial waste into valuable industrial products. However, the intricate reaction mechanism and rate-limiting process remain inadequately elucidated. The suitable reaction conditions should be clarified when designing industrial produce.

Effects of Diets With Different Carbohydrate to Lipid Ratios on the Growth Performance, Ion Transport, and Carbohydrate, Lipid and Ammonia Metabolism of Nile Tilapia () Under Long-Term Saline-Alkali Stress.

A 50-day test was adopted to compare the growth performance, liver histology, glucose metabolism, lipid (L) metabolism, ion transport, and ammonia metabolism of tilapia fed different carbohydrate-lipid (C:L) ratio diets under saline-alkaline water (salinity = 16 mmol/L and alkalinity = 35 mmol/L). The C and L levels of five isoenergetic (16.5 kJ/g) and isonitrogenous (32% protein) diets were C45%:L3% (L3), C38%:L6% (L6), C31%:L9% (L9), C24%:L12% (L12), and C17%:L15% (L15).

Proline metabolism is essential for alkaline adaptation of Nile tilapia (Oreochromis niloticus).

Background: Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus).

Molecular pathways of osmoregulation in response to salinity stress in the gills of the scalloped spiny lobster (Panulirus homarus) within survival salinity.

Scalloped spiny lobster (Panulirus homarus) aquaculture is the preferred strategy to resolve the conflict between supply and demand for lobster. Environmental conditions, such as salinity, are key to the success of lobster aquaculture. However, physiological responses of P.

Developmental toxicity of the neonicotinoid pesticide clothianidin to the larvae of the crustacean Decapoda, Penaeus vannamei.

The developmental toxicity effects of neonicotinoid pesticides such as clothianidin have not been fully explored in agricultural applications. This is particularly noteworthy because such pesticides significantly impact the survival rates of invertebrates, with arthropod larvae being particularly vulnerable. This study aimed to address this research gap by specifically investigating the toxicological effects of clothianidin on the developmental stages of the larvae of the economically important aquaculture species Penaeus vannamei.

Next-generation neonicotinoid: The impact of cycloxaprid on the crustacean decapod Penaeus vannamei.

Cycloxaprid, a new neonicotinoid pesticide, poses ecological risks, particularly in aquatic environments, due to its unique action and environmental dispersal. This study investigated the ecotoxicological effects of various concentrations of cycloxaprid on Penaeus vannamei over 28 days. High cycloxaprid levels significantly altered shrimp physiology, as shown by changes in the hepatosomatic index and fattening.

Safety evaluation and effects of dietary phlorotannins on the growth, health, and intestinal microbiota of Litopenaeus vannamei.

Phlorotannins are phenolic compounds with diverse biological activities, yet their efficacy in aquatic animals currently remains unclear. This investigation scrutinized the influence of phlorotannins on the growth, immunity, antioxidant capacity, and intestinal microbiota in Litopenaeus vannamei, concurrently evaluating the potential adverse effects of phlorotannins on L. vannamei.

Dinotefuran exposure alters biochemical, metabolomic, gut microbiome, and growth responses in decapoda pacific white shrimp Penaeus vannamei.

Dinotefuran, a neonicotinoid insecticide, may impact nontarget organisms such as Decapoda P. vannamei shrimp with nervous systems similar to insects. Exposing shrimp to low dinotefuran concentrations (6, 60, and 600 μg/L) for 21 days affected growth, hepatosomatic index, and survival.

The interaction between lipid and vitamin D impacts lipid metabolism and innate immunity in Chinese mitten crabs Eriocheir sinensis.

As a fat-soluble vitamin, vitamin D relies on fat to perform its biological function, affecting lipid metabolism and innate immunity. This study used different percentages of lipid and vitamin D diets to evaluate the synergistic effects on the growth, lipid metabolism and immunity of juvenile Eriocheir sinensis (5.83 ± 0.

Effects of myo-inositol on growth and biomarkers of environmental stress and metabolic regulation in Pacific white shrimp (Litopenaeus vannamei) reared at low salinity.

This study explored the role of myo-inositol in alleviating the low salinity stress of White Shrimp (Litopenaeus vannamei). Juvenile shrimp (0.4 ± 0.

Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, .

The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, (initial weight 11.52 ± 0.23 g).

Quantitative proteomic and metabolomic profiling reveals different osmoregulation mechanisms of tilapia cells coping with different hyperosmotic stress.

This study aimed to investigate the different regulatory mechanisms of euryhaline fish under regular hyperosmotic and extreme hyperosmotic stress. The OmB (Oreochromis mossambicus brain) cells were exposed to three treatments: control, regular hyperosmotic stress and extreme hyperosmotic stress. After 12 h exposure, proteomics, metabolomics analyses and integrative analyses were explored.

Study on the osmotic response and function of -inositol oxygenase in euryhaline fish nile tilapia ().

To understand the role of -inositol oxygenase () in the osmotic regulation of Nile tilapia, its expression was analyzed in various tissues. The results showed that the expression of gene was highest in the kidney, followed by the liver, and was significantly upregulated in the kidney and liver under 1 h hyperosmotic stress. The relative luminescence efficiency of the gene transcription starting site (-4,617 to +312 bp) under hyperosmotic stress was measured.

Growth and Hepatopancreas Health of Juvenile Chinese Mitten Crab () Fed Different Levels of Black Soldier Fly () Larvae Meal for Fish Meal Replacement.

A 56-day feeding trial assessed the effects of black soldier fly larvae meal (BSFLM) on the growth performance and hepatopancreas health of juvenile . Six isoproteic and isolipidic diets with 0% (FM), 10% (BSFLM10), 20% (BSFLM20), 30% (BSFLM30), 40% (BSFLM40), or 50% (BSFLM50) replacement of fish meal by BSFLM were formulated. Compared to FM, replacing 10%-40% of fish meal with BSFLM did not significantly affect the weight gain rate (WGR) or specific growth rate (SGR), while BSFLM50 significantly decreased the WGR and SGR.

Dietary β-Glucan Alleviates Antibiotic-Associated Side Effects by Increasing the Levels of Antioxidant Enzyme Activities and Modifying Intestinal Microbiota in Pacific White Shrimp ().

Antibiotics and their secondary metabolites are commonly found in aquatic ecosystems, leading to the passive exposure of many aquatic animals to low doses of antibiotics, which can affect their health. However, there is limited information available on how to mitigate the side effects of antibiotics on normal aquatic animals. This study aimed to investigate the potential of dietary β-glucan to alleviate the side effects induced by antibiotics in Pacific white shrimp () (0.

Identification of key genes and molecular pathways associated with claw regeneration in mud crab (Scylla paramamosain).

The mud crab (Scylla paramamosain) possesses extensive regenerative abilities, enabling it to replace missing body parts, including claws, legs, and even eyes. Studying the genetic and molecular mechanisms underlying regenerative ability in diverse animal phyla has the potential to provide new insights into regenerative medicine in humans. In the present study, we performed mRNA sequencing to reveal the genetic mechanisms underlying the claw regeneration in mud crab.

Effects of Dietary β-Glucan Feeding Strategy on the Growth, Physiological Response, and Gut Microbiota of Pacific White Shrimp, , under Low Salinity.

An eight-week feeding trial was conducted to investigate the effects of a dietary β-glucan application strategy on the growth performance, physiological response, and gut microbiota of Pacific white shrimp () (0.49 ± 0.17 g) under low salinity.

Reducing Dietary Protein Content by Increasing Carbohydrates Is More Beneficial to the Growth, Antioxidative Capacity, Ion Transport, and Ammonia Excretion of Nile Tilapia () under Long-Term Alkalinity Stress.

Alkalinity stress is the main stress experienced by aquatic animals in saline-alkali water, which hinders the aquaculture development and the utilization of water resources. The two-factor (2 × 3) test was adopted to study the influence of dietary protein to carbohydrate ratios on the energy metabolism of Nile tilapia () under different alkalinity stress levels. Three diets with different protein-carbohydrate ratios (P27/C35, P35/C25, and P42/C15) were fed to fish cultured in freshwater (FW, 1.

Identifying genes involved in the secretory physiological response to feeding in Pacific White Shrimp (Litopenaeus vannamei) using transcriptomics.

The physiological response to feeding is important for production aspects that include feed utilization and growth, and the responses require the action of numerous secretory factors. However, as an important aquaculture animal, the secretory response of Pacific White Shrimp (Litopenaeus vannamei) after feeding has not been comprehensively characterized. In this study, transcriptome analysis showed that 3172 differentially expressed genes were involved in the post-feeding response, including 289 new genes not annotated in the L.

Identification of key overlapping DEGs and molecular pathways under multiple stressors in the liver of Nile tilapia (Oreochromis niloticus).

The identification of key genes and molecular pathways that are involved in the response to stressors is crucial for controlling stress in fish and sustainable aquaculture. Environmental stressors can induce stress responses in aquatic animals, resulting in compromised immune function, inhibited growth, and increased mortality rates. mRNA-seq analysis provides a powerful tool to identify key genes and pathways associated with stress response.