Zhonghua Yan Ke Za Zhi
June 2006
Objective: To develop and set up a new culture system, which can apply pressure to cultured cells with open cycling air. The effects of this new system on the pH value, HCO(3)(-) concentration, O(2) pressure (pO(2)), CO2 pressure (pCO(2)) and the proliferation of retinal pigment epithelium (RPE) were tested to evaluate its efficiency in the study of glaucoma.
Methods: In the open cycling air pressure control culture system, the pressure inside the culture flasks was controlled by increase or decrease of the perfuse airflow.
Objective: To measure the number of glucocorticoid receptors (GR) in the trabecular meshwork and peripheral blood lymphocytes of rabbits, and to explore their relationship with steroid-induced glaucoma.
Methods: Steroid-induced glaucoma model was induced by subconjunctival injection of 0.5 mg dexamethasone in the right eyes every two days for a month.