The health equity mandate.

People of color and the poor die younger than the White and prosperous. And when they are alive, they are sicker. Health inequity is morally tragic.

The KdpD Sensor Kinase of Escherichia coli Responds to Several Distinct Signals To Turn on Expression of the Kdp Transport System.

Unlabelled: Kdp, one of three saturable K(+) uptake systems in Escherichia coli, is the system with the highest affinity for K(+) and the only one whose expression is strongly controlled by medium K(+) concentration. Expression is controlled by a two-component system of KdpD, the sensor kinase, and KdpE, the response regulator. There is general agreement that expression occurs when the growth rate of cells begins to become limited by K(+) availability.

Calculation of the three-dimensional structures of two antigenic sequences, pro-pro-pro-pro-asn-pro-asn-asp-pro and pro-pro-pro-pro-asn-pro-asn-asp-pro-pro-pro, of the circumsporozoite protein from a malaria-causing Plasmodium.

Using the chain build-up procedure based on the program ECEPP, we have computed the lowest energy structures for two terminally blocked subsequences from the antigenic circumsporozoite protein of Plasmodium berghei, that is known to cause malaria in animals. The full antigenic sequence is an octapeptide proline-rich tandem repeat, (Pro-Pro-Pro-Pro-Asn-Pro-Asn-Asp)(2). We computed the structures for the first octapeptide plus one Pro from the second octapeptide, terminally blocked CH(3)CO-Pro-Pro-Pro-Pro-Asn-Pro-Asn-Asp-Pro-NHCH(3) as well as the first octpeptide with an additional three Pro residues from the adjoining unit, i.

When and how are spatial perceptions scaled?

This research was designed to test the predictions of 2 approaches to perception. By most traditional accounts, people are thought to derive general-purpose spatial perceptions that are scaled in arbitrary, unspecified units. In contrast, action-specific approaches propose that the angular information inherent in optic flow and ocular-motor adjustments is rescaled and transformed into units related to intended actions.

Pseudolymphoma evolving into diffuse large B-cell lymphoma.

A 46-year-old man presented with a 1-year history of asymptomatic papules on the right arm, without an antecedent event. Initial clinical and histopathologic features were consistent with a pseudolymphoma without gene rearrangements, and the patient was treated with intralesional glucocorticoids. Four months later, the patient developed additional papules and plaques on the right arm, and, at this time, clinical and histopathologic features were most consistent with a T-cell-rich, large B-cell lymphoma, with monoclonal immunoglobulin light chain gene rearrangement.

Distances appear different on hills.

When walking effort is increased due to manipulations such as wearing heavy backpacks, people perceive hills to be steeper and distances to be farther (Bhalla & Proffitt, 1999; Proffitt, Stefanucci, Banton, & Epstein, 2003). On the basis of these findings, we expected people to overestimate distances on steep hills relative to the same distances on flat ground, because of the increased effort required to ascend or descend them. This hypothesis is in contrast to the belief that distances are specified solely by optical and oculomotor information related to the geometry of the environment.

Tool use affects perceived distance, but only when you intend to use it.

Recent research demonstrates neurologic and behavioral differences in people's responses to the space that is within and beyond reach. The present studies demonstrated a perceptual difference as well. Reachability was manipulated by having participants reach with and without a tool.

Cutaneous sarcoidosis.

Sarcoidosis, a multisystem disease of unknown etiology, remains enigmatic in modern medicine. Skin involvement happens in < 20% of patients with this disease. Yet, when it occurs, it can be virtually diagnostic or it can be so baffling and obtuse as to test the diagnostic skills of virtually any clinical dermatologist.

Perceiving distance: a role of effort and intent.

Perceiving egocentric distance is not only a function of the optical variables to which it relates, but also a function of people's current physiological potential to perform intended actions. In a set of experiments, we showed that, as the effort associated with walking increases, perceived distance increases if the perceiver intends to walk the extent, but not if the perceiver intends to throw. Conversely, as the effort associated with throwing increases, perceived distance increases if people intend to throw to the target, but not if they intend to walk.

Multiple paths for nonphysiological transport of K+ in Escherichia coli.

Mutants of Escherichia coli lacking all of the known saturable K+ transport systems, "triple mutants," require elevated K+ concentrations for growth. K+ transport activity in such mutants, called TrkF activity, has low substrate specificity and a low rate that increases with increasing external pH. Attempts to isolate mutants requiring even higher concentrations of K+ failed, implying that either TrkF is essential or is composed of multiple minor K+ transport activities.

The roles and regulation of potassium in bacteria.

Potassium is the major intracellular cation in bacteria as well as in eucaryotic cells. Bacteria accumulate K+ by a number of different transport systems that vary in kinetics, energy coupling, and regulation. The Trk and Kdp systems of enteric organisms have been well studied and are found in many distantly related species.

The role of effort in perceiving distance.

Berkeley proposed that space is perceived in terms of effort. Consistent with his proposal, the present studies show that perceived egocentric distance increases when people are encumbered by wearing a heavy backpack or have completed a visual-motor adaptation that reduces the anticipated optic flow coinciding with walking effort. In accord with Berkeley's proposal and Gibson's theory of affordances, these studies show that the perception of spatial layout is influenced by locomotor effort.

Internalization: a metaphor we can live without.

Shepard has supposed that the mind is stocked with innate knowledge of the world and that this knowledge figures prominently in the way we see the world. According to him, this internal knowledge is the legacy of a process of internalization; a process of natural selection over the evolutionary history of the species. Shepard has developed his proposal most fully in his analysis of the relation between kinematic geometry and the shape of the motion path in apparent motion displays.

Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli.

Mechanosensitive channels play an essential role in the regulation of turgor pressure in bacteria. In Escherichia coli, there are multiple mechanosensitive channels that have been characterized genetically: MscL, YggB and KefA. In this report, we describe the cloning of the kefA gene, the organization of the KefA protein and the phenotype of a missense mutation, kefA, which affects the KefA mechanosensitive channel.

The monoterpenes of Artemisia tridentata ssp. vaseyana, Artemisia cana ssp. viscidula and Artemisia tridentata ssp. spiciformis.

Monoterpenes from three different members of the Anthemideae family, Artemisia tridentata ssp. vaseyana, Artemisia cana ssp. viscidula and Artemisia tridentata ssp.

Modulation of KdpD phosphatase implicated in the physiological expression of the kdp ATPase of Escherichia coli.

The KdpD sensor kinase and the KdpE response regulator control the expression of the kdpFABC operon, encoding the KdpFABC high-affinity K+ transport system of Escherichia coli. Low turgor pressure has been postulated to be the environmental stimulus to express KdpFABC. KdpD has autokinase, phosphotransferase and, like many sensor kinases, response regulator (phospho-KdpE) specific phosphatase activity.

Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression scanning mutagenesis.

The Kdp-ATPase of Escherichia coli is a four-subunit P-type ATPase that accumulates K(+) with high affinity and specificity. Residues clustered in four regions of the KdpA subunit of Kdp were implicated as critical for K(+) binding from the analysis of mutants with reduced affinity for K(+) (Buurman, E., Kim, K.

Replacement of glycine 232 by aspartic acid in the KdpA subunit broadens the ion specificity of the K(+)-translocating KdpFABC complex.

Replacement of glycine residue 232 with aspartate in the KdpA subunit of the K(+)-translocating KdpFABC complex of Escherichia coli leads to a transport complex that has reduced affinity for K(+) and has lost the ability to discriminate Rb(+) ions (, J. Biol. Chem.

Treatment of severe atopic dermatitis by topical immune modulation using dinitrochlorobenzene.

We present the results of a small uncontrolled pilot trial which suggest that contact sensitization to dinitrochlorobenzene and repeated weekly applications significantly improve the clinical status of severe atopic dermatitis in adults. Although no changes were noted in circulating levels of either lymphocyte subset populations or the serum cytokines assayed in this trial, our observations may be due to topical immune modulation by dinitrochlorobenzene. Larger controlled studies of dinitrochlorobenzene treatment in atopic dermatitis are warranted.

The Kdp-ATPase of Escherichia coli mediates an ATP-dependent, K+-independent electrogenic partial reaction.

Charge transport by the K+ transporting Kdp-ATPase from Escherichia coli was investigated using planar lipid membranes to which liposomes reconstituted with the enzyme were adsorbed. To study reactions in the absence of K+, given some contamination of solutions with K+, we used a mutant of Kdp whose affinity for K+ was 6 mM instead of the wild-type whose affinity is 2 microM. Upon rapid release of ATP from caged ATP, a transient current occurred in the absence of K+.

Colorimetric determination of citrulline residues in proteins.

A method is described for the direct colorimetric determination of citrulline residues in proteins based on the reaction with diacetylmonoxime in the presence of lower concentrations of sulfuric acid. The reduced sensitivity due to the lower acid concentration was overcome by the addition of ferric chloride which also contributed to the color stabilization. Insoluble proteins or proteins resulting in turbidity can be analyzed following partial hydrolysis of those with enzyme or acid.

Assembly of the Kdp complex, the multi-subunit K+-transport ATPase of Escherichia coli.

Kdp, the high affinity ATP-driven K+-transport system of Escherichia coli, is a complex of the membrane-bound subunits KdpA, KdpB, KdpC and the small peptide KdpF. The assembly of this complex was studied by the analysis of mutants that expressed two of the three large subunits and inserted them into the cytoplasmic membrane. In the strains that do not express KdpC or KdpA the other two subunits did not copurify on dye-ligand affinity columns after solubilization with non-ionic detergent.