-mediated methionine deprivation drives immune activation and enhances immune checkpoint blockade therapy in melanoma.

Background: Although immune checkpoint inhibitor (ICI)-based therapy is advantageous for patients with advanced melanoma, resistance and relapse are frequent. Thus, it is crucial to identify effective drug combinations and develop new therapies for the treatment of melanoma. SGN1, a genetically modified species that causes the targeted deprivation of methionine in tumor tissues, is currently under investigation in clinical trials.

[Determination of 14 aniline and benzidine compounds in soil by gas chromatography-mass spectrometry].

The complex matrix of soil samples and low extraction efficiency of aniline compounds limit many methods developed for detecting aniline and benzidine compounds in soil. In this study, a rapid and sensitive method based on gas chromatography-mass spectrometry was developed for the simultaneous determination of 14 aniline and benzidine compounds in soil. The collected soil samples were sealed with 5% sodium sulfite solution and refrigerated to inhibit the oxidation of the target compounds for up to 7 d.

[Determination of polycyclic aromatic hydrocarbons in airborne particles by microwave extraction-high performance liquid chromatography].

A method by using microwave extraction and high performance liquid chromatography was developed to determine polycyclic aromatic hydrocarbons (PAHs) in airborne particles. The glass/quartz fiber filter, on which particles adsorbed, was extracted with a mixed solvent -hexane/acetone (1:1, v/v) using microwave, and the subsequent purification of the extract was carried out by a florisil cartridge. The prepared sample was determined by high performance liquid chromatography coupled with photodiode array (PDA) and fluorescence dual-detector.

[Determination of trace and ultra-trace level bromate in water by large volume sample injection with enrichment column for on-line preconcentration coupled with ion chromatography].

A method for the determination of trace and ultra-trace level bromate in water by ion chromatography with large volume sample injection for on-line preconcentration was established. A high capacity Dionex IonPac AG23 guard column was simply used as the enrichment column instead of the loop for the preconcentration of bromate. High purity KOH solution used as eluent for gradient elution was on-line produced by an eluent generator automatically.

[Determination of 21 phenolic compounds in soil by ultrasonic extraction-gas chromatography].

An effective method was developed to determine phenolic compounds in soil by ultrasonic extraction-gas chromatography (GC). The soil sample was extracted with methylene dichloride-hexane (2:1, v/v) for 3 min with the extraction temperature lower than 50 degrees C for 3 times. The extract was purified by alkaline aqueous solution (pH > 12), and the phenolic compounds were dissolved in aqueous solution.

[Determination of seven nitrobenzene compounds in water samples by single-drop microextraction with capillary gas chromatography].

A method suitable for the extraction of nitrobenzene, nitrotoluenes and nitrochlorobenzenes from water samples using single-drop microextraction with capillary gas chromatography is presented. Parameters, such as organic solvents, drop volume of organics, agitation, immerge depth of needle point, aqueous sample volume, exposure time and temperature were controlled and optimized. The developed protocol was found to yield a linear calibration curve in a concentration range from 0.

[Determination of trace phenol compounds using gas chromatography-mass spectrometry coupled with Tenax adsorption tube for enrichment of air samples].

A novel determination method for trace of seven phenol compounds in air samples has been established. They were collected with Tenax adsorption tube (180 mm x 60 mm glass tube packed with 150 mg Tenax (40 - 60 mesh)) and desorbed with methanol. Five microlitres of naphthalene-D8 (internal standard) solution was added to the eluate.