Lightweight tea bud detection method based on improved YOLOv5.

Tea bud detection technology is of great significance in realizing automated and intelligent plucking of tea buds. This study proposes a lightweight tea bud identification model based on modified Yolov5 to increase the picking accuracy and labor efficiency of intelligent tea bud picking while lowering the deployment pressure of mobile terminals. The following methods are used to make improvements: the backbone network CSPDarknet-53 of YOLOv5 is replaced with the EfficientNetV2 feature extraction network to reduce the number of parameters and floating-point operations of the model; the neck network of YOLOv5, the Ghost module is introduced to construct the ghost convolution and C3ghost module to further reduce the number of parameters and floating-point operations of the model; replacing the upsampling module of the neck network with the CARAFE upsampling module can aggregate the contextual tea bud feature information within a larger sensory field and improve the mean average precision of the model in detecting tea buds.

Spatiotemporal dynamics and spatial correlation patterns of urban ecological resilience across the Yellow River Basin in China.

Addressing the need to harmonize environment conservation and sustainable economic development within the Yellow River Basin (YRB) requires a profound comprehension of the spatiotemporal dynamics of urban ecosystem resilience. This study developed an index system utilizing the resistance-adaptability-recovery framework to measure these dynamics. By applying the advanced multi-attribute boundary area comparison method and a spatial autocorrelation model, we investigated the spatiotemporal variations and spatial correlation patterns of urban ecological resilience across the YRB.

Can rewards enhance creativity? Exploring the effects of real and hypothetical rewards on creative problem solving and neural mechanisms.

Reward cues have long been considered to enhance creative performance; however, little is known about whether rewards can affect creative problem solving by manipulating states of flexibility and persistence. This study sought to elucidate the differential impacts of real versus hypothetical rewards on the creative process utilizing the Chinese compound remote association task. Behavioral analysis revealed a significantly enhanced solution rate and response times in scenarios involving real rewards, in contrast to those observed with hypothetical rewards.

Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma.

Background: Cutaneous melanoma is one of the most invasive and lethal skin malignant tumors. Compared to primary melanoma, metastatic melanoma (MM) presents poorer treatment outcomes and a higher mortality rate. The tumor microenvironment (TME) plays a critical role in MM progression and immunotherapy resistance.

Comorbidities, drug-resistance and length of hospital stay among tuberculosis inpatients in Northeastern China: a retrospective observational study from 2013 to 2021.

Background: Tuberculosis (TB) remains a significant global health issue. Drug-resistant TB and comorbidities exacerbate its burden, influencing treatment outcomes and healthcare utilization. Despite the growing prevalence of TB comorbidities, research often focuses on single comorbidities rather than comorbidity patterns.

The relationship between serum uric acid and homocysteine is influenced by kidney function.

Background And Aim: Prior research has established a relationship between homocysteine (Hcy) and serum uric acid (SUA) levels; however, the precise mechanisms underlying this association remain unclear. The objective of this research was to explore the correlation between SUA and Hcy and to evaluate the possible role of kidney function as a mediator in the connection between SUA and Hcy.

Methods And Results: Consecutive enrollment of 16870 participants aged 20-60 years was conducted at the Third Xiangya Hospital of Central South University.

Disrupted brain networks underlying high-fidelity memory retrieval in subjective cognitive decline: A task-based fMRI study.

Introduction: Subjective cognitive decline (SCD) is linked to memory complaints and disruptions in certain brain regions identified by molecular imaging and resting-state functional magnetic resonance imaging studies. However, it remains unclear how these regions interact to contribute to both subjective and potential objective memory issues in SCD.

Methods: To address this gap, task-based imaging studies are essential.

Mitigation of irreversible membrane biofouling by CNTs-PVDF conductive composite membrane.

Biofouling has been one of the major challenges impacting the long-term stable operation of ultrafiltration processes. Irreversible biofouling is considerably more harmful than reversible biofouling. Conductive membrane, as a new technology to effectively mitigate membrane fouling, lack research of controlling irreversible biofouling.

Comprehensive analysis of heterogeneity and cell-cell interactions in Crohn's disease reveals novel location-specific insights.

Introduction: In Crohn's disease (CD), lesions are mainly distributed in a segmental manner, with the primary sites of involvement being the ileum and colon. Heterogeneity in colon and ileum results in location-specific clinical presentations and therapeutic responses. Mucosal healing tends to be more readily and quickly achieved in the colon than in the ileum, where lesions are more likely to develop into complex behaviors.

Enhanced flame retardancy and accelerated degradation of polylactic acid using a chitosan-based additive with phosphorus and zinc.

Polylactic acid (PLA) is a bio-recyclable plastic, but its high flammability limits broader applications. Here, a novel flame retardant (Zn-CHP) is synthesized from chitosan (CH), diethylenetriaminepenta (methylenephosphonic) acid (DTPMP), and ZnCl₂ using a simple, solvent-free process. The Zn-CHP additive is melt-blended with PLA, achieving excellent flame retardancy at just 2 wt% loading.

Immobilization of snailase and β-glucosidase on L-aspartic acid-modified magnetic amorphous ZIF for efficiently and sustainably producing ginsenoside compound K.

Improving the catalytic efficiency and recyclability of immobilized enzyme remained a serious challenge in industrial applications. Enzyme immobilization in the amorphous zeolite imidazolate framework (aZIF) preserved high enzyme activity, but still faced separation difficulties and a low catalytic efficiency in practice. In this study, a one-pot co-precipitation method was used to form the enzyme-aZIF/magnetic nanoparticle (MNP) biocomposite by rapidly precipitating snailase (Sna) and β-glucosidase (β-G) with metal/ligand on MNP and modifying with L-aspartic acid (Asp).

Immobilization of β-glucosidase and β-xylosidase on inorganic nanoparticles for glycosylated substances conversion.

There are abundant glycosylated substances such as cellulose, hemicellulose, and phytochemical glycosides in plants, which could be converted into functional chemicals such as monosaccharides, oligosaccharides, and bioactive aglycones by cleavage of glycosidic bonds using glycoside hydrolases (GHs). Among those GHs, β-glucosidase and β-xylosidase are the rate-limiting enzymes for degrading cellulose and hemicellulose, respectively, and can convert a variety of glycosylated substances. These two enzymes play important roles in the high value use of plant resources and have great potential applications.

Effects of mirror-image nucleosides on DNA replication and transcription in human cells.

Mirror-image nucleosides, as potential antiviral drugs, can inhibit virus DNA polymerase to prevent virus replication. Conversely, they may be inserted into the DNA strands during DNA replication or transcription processes, leading to mutations that affect genome stability. Accumulation of significant mutation damage in cells may result in cell aging, apoptosis, and even uncontrolled cell division.

Histone N-tails modulate sequence-specific positioning of nucleosomes.

Spatial organization of chromatin is essential for cellular functioning. However, the precise mechanisms governing sequence-dependent positioning of nucleosomes on DNA still remain unknown in detail. Existing algorithms, taking into account the sequence-dependent deformability of DNA and its interactions with the histone globular domains, predict rotational setting of only 65% of human nucleosomes mapped in vivo.

Novel peptide inhibitor of matrix Metalloproteinases-1 from pufferfish skin collagen hydrolysates and its potential Photoprotective activity via the MAPK/AP-1 signaling pathway.

Takifugu bimaculatus, a pufferfish species farmed in Fujian Province, is known for its non-toxic flesh and collagen-rich skin. We identified a novel collagen-derived matrix metalloproteinase 1 (MMP-1) inhibitory peptide, from T. bimaculatus skin with potent anti-photoaging properties.

Comparative analysis of sensory properties and chemical composition in grape spirits: Pervaporation separation vs. distillation.

To clarify the effects of pervaporation and distillation on aroma profiles, the Sensomics approach investigated the aroma characteristics and key aroma compounds of Cabernet Sauvignon (CS) and Ugni Blanc (UB) grape spirits produced by pervaporation (UB-P, CS-P) and distillation (UB-D, CS-D). The results indicated that pervaporated grape spirits exhibited stronger floral and fruity aromas, while distilled grape spirits were characterized by more pronounced cooked apple and toasty aromas. Consumers preferred products with intense floral and fruity aromas and weaker cooked apple note.

Anlotinib enhances the pro-apoptotic effect of APG-115 on acute myeloid leukemia cell lines by inhibiting the P13K/AKT signaling pathway.

Background: APG-115 is a novel small-molecule selective inhibitor that destabilizes the p53-MDM2 complex and activates p53-mediated apoptosis in tumor cells. Anlotinib inhibits tumor angiogenesis and promotes apoptosis. In this study, we investigated the apoptotic effect and potential mechanism of APG-115 and anlotinib combination on AML cell lines with different p53 backgrounds.

HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis.

Advanced atherosclerosis is the pathological basis for acute cardiovascular events, with significant residual risk of recurrent clinical events despite contemporary treatment. The death of foamy macrophages is a main contributor to plaque progression, but the underlying mechanisms remain unclear. Bulk and single-cell RNA sequencing demonstrated that massive iron accumulation in advanced atherosclerosis promoted foamy macrophage ferroptosis, particularly in low expression of triggering receptor expressed on myeloid cells 2 (TREM2) foamy macrophages.

Comparative chloroplast genome analyses provide new insights into molecular markers for distinguishing Arnebiae Radix and its substitutes (tribe Lithospermeae, Boraginaceae).

Background: Arnebiae Radix has long been used in traditional medicine for its pleiotropic properties. However, distinguishing Arnebiae Radix from its substitutes or closely related species has been challenging due to limited phenotypic characteristics.

Purpose: We aimed to identify the molecular markers for distinguishing Arnebiae Radix from its confusion species.

Exploring the gut microbiota and metabolome of Lateolabrax japonicus: A multi-omics approach.

The intestinal microbiota plays a crucial role in the health and development of fish, engaging in intricate interactions with the host organism. As a significant species in aquaculture, Lateolabrax japonicus serves as an exemplary model for investigating these interactions and their subsequent effects on growth and health. This study utilized a multi-omics approach, incorporating metagenomic sequencing and non-targeted metabolomics, to delineate the gut microbiota and metabolome of L.

One-Pot Synthesis of Oxygen Vacancy-Rich Amorphous/Crystalline Heterophase CaWO Nanoparticles for Enhanced Radiodynamic-Immunotherapy.

Radiodynamic therapy that employs X-rays to trigger localized reactive oxygen species (ROS) generation can tackle the tissue penetration issue of phototherapy. Although calcium tungstate (CaWO) shows great potential as a radiodynamic agent benefiting from its strong X-ray absorption and the ability to generate electron-hole (e-h) pairs, slow charge carrier transfer and fast e-h recombination greatly limit its ROS-generating performance. Herein, via a one-pot wet-chemical method, oxygen vacancy-rich amorphous/crystalline heterophase CaWO nanoparticles (Ov-a/c-CaWO NPs) with enhanced radiodynamic effect are synthesized for radiodynamic-immunotherapy of cancer.

The OsMAPK6-OsWRKY72 module positively regulates rice leaf angle through brassinosteroid signals.

Leaf angle is a major agronomic trait that determines plant architecture, which directly affects rice planting density, photosynthetic efficiency, and yield. The plant phytohormones brassinosteroids (BRs) and the MAPK signaling cascade are known to play crucial roles in regulating the leaf angle, but the underlying molecular mechanisms are not fully understood. Here, we report a rice WRKY family transcription factor gene, OsWRKY72, which positively regulates leaf angle by affecting lamina joint development and BR signaling.

Highly Efficient Analysis on Biomass Carbohydrate Mixtures by DREAMTIME NMR Spectroscopy.

Proton (H) NMR spectroscopy presents a powerful tool for biomass mixture studies by revealing the involved chemical compounds with identified ingredients and molecular structures. However, conventional H NMR generally suffers from spectral congestion when measuring biomass mixtures, particularly biomass carbohydrate samples, that contain various physically and chemically similar compounds. In this study, a targeted detection NMR approach, DREAMTIME, is exploited for studying biomass carbohydrate mixtures by spectroscopically targeting the desired compounds in separate 1D NMR spectra.

Tranexamic acid in hip and spine surgery for children with cerebral palsy - a PRISMA-compliant scoping review.

Many children with cerebral palsy (CP) are frail and require major hip and/or spine surgeries associated with substantial blood loss. Tranexamic acid (TXA) is commonly used to reduce blood loss, but there is uncertainty around the optimal dose and timing of administration. There have been reviews in sub-populations and specific dosing regimens, but a broad overview of the available literature is lacking.