Zhongguo Shi Yan Xue Ye Xue Za Zhi
October 2024
Objective: To investigate the molecular mechanism and distribution characteristics of RhD negative phenotypes in Han population of blood donors in Wuhu city.
Methods: A total of 210 RhD samples from August 2021 to August 2022 were screened by serological test and collected from Wuhu Central Blood Station for the voluntary blood donor population. Exons 1 and 10 of the gene were amplificated by PCR to determine whether the samples had the gene.
Dermatophagoides farinae (Acari: Pyroglyphidae) has been reported as one of the major sources of indoor allergens that trigger allergic disease in humans. In this study, the genetic diversity and differentiation of nine geographic populations of D. farinae were investigated by analyzing mitochondrial and nuclear genes (COI, Cytb, COI+Cytb, and ITS).
View Article and Find Full Text PDFBioinformatics methods were used to analyze the role of PCAT6 in a variety of tumors and verify its role in oesophageal squamous cell carcinoma (ESCC) EC109 cells. The pan-cancer dataset was downloaded from the University of California Santa Cruz (UCSC) database to analyze the expression of PCAT6 in pan-cancer and its relationship with prognosis, clinical features, and immune infiltration. The expression and prognosis of PCAT6 in ESCC were verified by Gene Expression Omnibus (GEO) and Kaplan-Meier database.
View Article and Find Full Text PDFAstigmatid mites are economically significant pests of stored products and sources of inhalant allergens causing allergic rhinitis and asthma worldwide. The morphological identification of astigmatid mites at the species level is often a difficult task due to their small size, phenotypic similarity and lack of diagnostic characters. We used multiplex polymerase chain reaction (PCR) to identify astigmatid mite species, which could complement the morphological data for the species-specific identification of mites.
View Article and Find Full Text PDFMites from the Acaroidea (Sarcoptiformes: Astigmatina) are important pests of various stored products, posing potential threats to preserved foods. In addition, mites can cause allergic diseases. Complete mitochondrial genomes (mitogenomes) are valuable resources for different research fields, including comparative genomics, molecular evolutionary analysis, and phylogenetic inference.
View Article and Find Full Text PDFmA is a widespread RNA modification. However, the mechanism through which mA regulated the progress of oesophageal squamous cell carcinoma (ESCC) remains undetermined. The levels and prognosis of WTAP were analysed using an ESCC tissue microarray (87 ESCC and 44 paracancerous tissues).
View Article and Find Full Text PDFOzobranchus jantseanus is the largest metazoan known to survive in liquid nitrogen without pretreatment to date; however, the mechanism underlying this tolerance remains unclear. In this study, the first analyses of histological and morphological changes in normal, frozen, and dehydrated states were performed. Adults survived after direct placement in liquid nitrogen for 96 h, with a survival rate of approximately 86.
View Article and Find Full Text PDFWe assembled and annotated the complete mitochondrial genome of . It is the first complete mitochondrial genome sequence from the genus . The mitogenome was 13,966 bp in length and contains 37 genes (including 13 protein-coding genes, 22 transfer RNA (tRNA), and two ribosomal RNA (rRNA)), and one largest non-coding region.
View Article and Find Full Text PDFBackground: The mitochondrial (mt) genomes of Sarcoptiformes mites typically contain 37 genes. Although the loss of genes is rare in Sarcoptiformes mite mitogenomes, two of the six previously reported oribatid mites (Acariforms: Sarcoptiformes) are reported to have lost parts of their tRNA genes. To confirm whether the tRNA genes were indeed lost and whether the loss is universal, we re-annotated the available oribatid mite sequences and sequenced the mitogenome of Oribatula sakamorii.
View Article and Find Full Text PDFBackground: Primary thyroid lymphoma (PTL) is a rare thyroid malignancy, there are few large sample studies on PTL and no standardized treatment regimen has been established due to the rarity. The aims of this study were to explore the incidence and prognostic factors of PTL and construct visual prognostic prediction models for post-chemotherapy and postoperative patients.
Methods: The incidence of PTL in 1975-2017 was extracted from the US Surveillance, Epidemiology, and End Results (SEER) database, then assessed using joinpoint regression software.
Anaplasma species are obligate intracellular pathogens that threaten the health of both humans and animals worldwide. This study aimed to determine the prevalence of Anaplasma species in stray dogs in Anhui Province, China. Blood samples from 201 apparently healthy stray dogs were collected from August 2017 to January 2018, and Anaplasma spp.
View Article and Find Full Text PDFIn this study, we de novo sequenced and analyzed the circular mitochondrial genome (mitogenome) of Tyrophagus putrescentiae. It was 14,156 bp long and contained a complete set of 37 genes, contrary to the initial published sequences; it included 22 tRNA sequences and the largest non-coding region. The mtDNA gene order of T.
View Article and Find Full Text PDFThe species of the genus Anaplasma are obligate intracellular pathogens that threaten the health of both humans and animals. In this study, we investigated the presence of Anaplasma phagocytophilum, A. ovis and A.
View Article and Find Full Text PDFEsophageal cancer (EC) is one of the most lethal cancers currently known. Members of the forkhead-box A (FOXA) family, including FOXA1 and FOXA2, have been reported to regulate EC progression. However, the role of FOXA3, which is another FOXA member, has not yet been investigated.
View Article and Find Full Text PDFZhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
May 2018
Our study reports a case of acarodermatitis caused by . By morphological observations, the mites seized were identified as (deutonymph) which could attack humans resulting in acarodermatitis characterized with the symptoms of papules and blisters in different degrees. The patient was treated with 15% calamine lotion and anti-inflammatory and antipruritic drugs.
View Article and Find Full Text PDFZhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
May 2017
Objective: To compare the effects of three kinds of RNA extraction methods, namely a modified SDS method, TRIzol reagent method, and CTAB method, so as to obtain an economical and efficient method for RNA extraction from .
Methods: The modified SDS method, TRIzol reagent method and CTAB method were applied to extract the RNA from . A nucleic acid protein analyzer was used to measure the concentration and purity of RNA.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
July 2017
Objective: To understand the natural population dynamics and spatial distribution of in storage flour, so as to provide an evidence for its prevention and control.
Methods: The samples from five sampling points in Wuhu City were collected monthly from January to December, 2013, and examined and counted for . The dispersion pattern target, Iwao's m*- ̄; regression analysis and Taylor's lg-lg ̄; regression analysis were used for analyzing the spatial distribution pattern of in the storage flour.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
April 2017
Objective: To optimize the extraction methods of mitochondrial genome DNA (mtDNA) of .
Methods: The pyrolysis, protein K variable-temperature digestion and high-concentration potassium acetate purification were applied to optimize the high-concentration-salt precipitation method, and then the optimized method was compared with two common extraction methods, the sucrose density gradient centrifugation method and traditional high-concentration-salt precipitation method. The mtDNA samples were identified by using spectrophotometry, agarose gel electrophoresis and the amplification products of COX1.
BACKGROUND Presently, studies of factors associated with drug-resistant tuberculosis (TB) focus on patients' socio-demographic characteristics and living habits, to the exclusion of biochemical indicators, especially immune factors. This study was carried out to determine whether immune factors are associated with drug-resistant TB. MATERIAL AND METHODS A total of 227 drug-resistant pulmonary TB patients and 225 drug-susceptible pulmonary TB patients were enrolled in this study.
View Article and Find Full Text PDFAllergen-induced airway inflammation is characterized by Th2-mediated eosinophilic inflammation in the lungs. While the molecular mechanisms leading to this abnormal Th2 response remain unclear. Recent studies have demonstrated that MicroRNAs (miRNAs) modulate allergic airway inflammation.
View Article and Find Full Text PDFContext: Recent studies show that the Agkistrodon acutus (Viperidae) (syn. Deinagkistrodon acutus) protein C activator (PCA) treats acute myocardial infarction and ischaemia-reperfusion animal models effectively, while the underlying mechanism remains unknown.
Objective: To study the effect of PCA on the injury of human umbilical vein endothelial cells (HUVECs) induced by HO and the underlying mechanism.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
June 2016
Objective: To characterize the population dynamics and spatial distribution of in the flour warehouse, so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitoring and management.
Methods: The samples from flour warehouses of four localities were collected, examined and counted for in every month in Wuhu City. The dispersion pattern target, Iwao / regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of in the flour warehouses.
Objective: To observe the and its hypopus in feed of .
Methods: Feed samples were collected from farms, and and its hypopus were separated from the samples by the vibration sieve method and floating method. The glass specimens were prepared and and its hypopus were observed under an optical microscope.