Sample Preparation and Fixation for Histology and Pathology.

The basic goal of all histological techniques is the identification of tissue components, both normal and pathologic. In the case of any immunodetection technique we expect: specific detection of the antigen with a mark strong enough to be easily detected; and a clear preservation of histological and cytological details of the environment. To achieve these expectations the technical procedures employed must be clearly understood so we can control the previous methodological steps, avoiding any failure and/or complication that could seriously affect the results of our study.

Epicutaneous Administration of 17β-Estradiol Induces Langerhans Cells Depletion.

Background: Langerhans cells (LC) number and function in mouse vaginal mucosa are affected by 17β-estradiol (E) application; nonetheless, its effect on epidermal LC has not been studied. The purpose of this study was to evaluate the effect of topical administration of E on the number, phenotype, and migratory ability of LC in mouse skin.

Methods: Ears of adult CD1 male mice were topically treated once with several doses.

Response to Infection by in a Murine Model.

Cardiopathy is a common, irreversible manifestation of the chronic phase of Chagas disease; however, there is controversy as to how the causes for progression from the acute to the chronic phase are defined. In this work, the presence of the parasite is correlated with the occurrence of cell infiltration and fibrosis in cardiac tissues, as well as IgG detection and disease progression in a murine model. Fifty CD1 mice were infected intraperitoneally with , while 30 control were administered with saline solution.

[Association between chronic urticaria and Helicobacter pylori].

Background: Chronic urticaria is a multifactorial disease classified in three main groups: mechanical, autoimmune and idiopathic. So far we don't know the mechanisms that produces it, there are different theories and one of them is the presence of Helicobacter pylori.

Objective: To establish the relation between chronic urticaria and Helicobacter pylori trough endoscopic and histopatological studies.