Metabolomic and secretomic approach to the resistance features of the fungus Aspergillus niger IOC 4687 to copper stress.

Metabolomic and secretomic analyses of Aspergillus niger IOC 4687 indicated the features of resistance of this strain to copper stress. To investigate the metabolites produced under oxidative stress conditions, gas chromatography-mass spectrometry analysis was performed. The secretome principal component analysis results showed that mannitol could be the main metabolite responsible for conferring resistance to the fungus, and gluconic acid is the possible cause of copper desorption because of its chelating ability.

Response mechanism of mine-isolated fungus Aspergillus niger IOC 4687 to copper stress determined by proteomics.

Proteomic analysis of the fungus Aspergillus niger showed that its capacity to absorb metals was boosted by physiological modification under metal stress conditions. To investigate the proteome elicited by copper stress, the mine-isolated strain A. niger IOC 4687 was cultured in the absence (control) or presence of copper ions (50 mg L) for 72 h.