Platelet aggregate ratios--standardization of technique and test results in patients with myocardial ischemia and patients with cerebrovascular disease.

The technique for measuring platelet aggregate ratios described by Wu and Hoak (1974) was evaluated in normal subjects. The following had no influence upon the test result: age, sex, fasting versu the postprandial state, and the degree of stasis prior to drawing the sample. Variance within subjects was small compared to variance between subjects (0.

Pharmacologic and hemodynamic influences on the rate of isovolumic left ventricular relaxation in the normal conscious dog.

We studied the effects of acute pharmacologic and hemodynamic interventions on isovolumic left ventricular relaxation in 19 conscious dogs using micromanometer tip catheters. Isoproterenol (11 studies) augmented peak rate of rise of left ventricular pressure [(+) dP/dt] by 1,275+/-227 (SE) mm Hg/s (P < 0.001) and dP/dt at an isopressure point of 35 mm Hg during isovolumic relaxation [(-) dP/dt(35)] by 435+/-80 mm Hg/s (P < 0.

[Immunochemical determination of human hemoglobin in biological fluids (author's transl)].

An assay of human hemoglobin by immunonephelometry-electroimmunodiffusion is proposed. Concentrations in the range from 5 to 180 mg% can be determined to within 2--5% accuracy. The immunonephelometric assay is independent of the presence of haptoglobin whereas the electroimmunodiffusion assay is not.

Immunochemical study on serum proteins in systemic sclerosis.

Forty one patients with systemic sclerosis were studied after separation into three groups according to Barnett's classification. A multi-dimensional statistical analysis eight serum proteins revealed a difference between control patients and patients with type I and type II scleroderma. Type I scleroderma was characterised by a rise in alpha 2 macroglobulin and in the C4 fraction of complement, whilst in type II scleroderma all the proteins studied were raised, with the exception of CO complement, which was normal, and transferrin which was markedly decreased.

Entomopathogens: ecological manipulation of natural associations.

The control of insect pests with entomopathogens is unique, in that naturally occurring host-pathogen relations are manipulated to the benefit of man: protecting agricultural crops and forests or controlling insect vectors of disease. The isolation and identification of a virulent pathogen is the initial step in the development of a potential control agent. Production of the pathogen in adequate quantities must be possible either in vivo (insects) or in vitro (artificial medium).

[Usual values of haptoglobin, protein-bound sialic acid and blood sedimentation in a population of the Cherbourg region].

The authors studied 3 parameters commonly used for the exploration of an inflammatory syndrome (determination of globular sedimentation rate at the first and second hour, concentration of plasma haptoglobin, sialic acid bound to plasma proteins rate). Haptoglobin and acid sialic assays were solely used in determining a control population (816 patients without any inflammatory reaction). As a matter of fact, the values we found complied with a normal distribution rule and confidence limits with a probability of 95% were obtained.

[Preparation and immunoephelometric quantitation of fibrinogen in rabbits].

Pure rabbit fibrinogen was prepared by a method involving two ammonium sulfate precipitations, one 2 M phosphate buffer precipitation, one DEAE cellulose chromatography and lastly one Sepharose 6 B chromatography. The aminoacid composition was determined and an immunonephelemetric assay was proposed. This assay followed an accurate determination of fibrinogen concentration in a rabbit with inflammatory reaction.

[Plasmatic glycoproteins and granulomatous reaction].

The first evolution phase of the inflammatory granuloma is characterized from biochemical point of view by an overproduction in the liver of some glycoproteins termed glycoproteins of the inflammatory reaction. The author comments the study of these glycoproteins and of the nature of the endogenic factor producing an increase of their plasmatic concentration. He discusses the physiopathologic significance of the overproduction of inflammatory reaction glycoproteins following the development of an experimental granuloma.

Biochemical criteria for the evaluation of drug efficiency on adjuvant arthritis and nephrotoxic serum nephritis in the rat: studies with phenylbutazone, L-Asparaginase, colchicine, lysine acetylsalicylate, and pyridinol carbamate.

The levels of serum orosomucoid, haptoglobin, and seromucoid were evaluated as possible quantitative criteria for the estimation of drug efficiency in adjuvant arthritis and nephrotoxic serum nephritis. In adjuvant arthritis, haptoglobin, seromucoid, and chiefly orosomucoid serum levels were generally very sensitive to anti-inflammatory agents such as phenylbutazone and pyridinol carbamate, and to immunosuppressive agents such as L-asparaginase. There was a significant correlation between the serum levels of these glycoproteins and the arthritis scores.

[Intravascular hemolysis. Assessment by determination of haptoglobin, hemopexin and orosomucoid].

In 100 patients suffering from intravascular haemolysis, a "protein profile" was made on the basis of measurement of haptoglobin, haemopexin and orosomucoid. The estimations were carried out using a rapid precise automatic technique using monospecific immune serum. The results obtained indicate that these tests are sensitive and reliable.

[An immunonephelometric determination of rabbit haptoglobin].

An immunonephelemetric determination of rabbit haptoglobin was carried out. At first, pure haptoglobin was isolated and then monospecific immunserums were obtained from rats. This method completes the regular determination of haptoglobin in all cases where the peroxydasic activity of the haptoglobin-hemoglobin complex is inhibited either by an hemolytic process, as is the case for rabbits, or by some inhibitors of the peroxydasic reaction.

[Haptoglobin assay using the LKB 8600 reaction rate analyzer].

The reaction rate analyzer LKB 8 600 is used in a new haptoglobin evaluation method. This technique involves formation of peroxidatic complex between haptoglobin and horse or human hemoglobin (human hemoglobin is easier to prepare), then oxidation of gaiacol with registering of optical density rise. Results fit completely with those of the classical Technicon autoanalyzer technique.