Analysis of protein expression profile of oral squamous cell carcinoma by MALDI-TOF-MS.

In this study, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF-MS) technology was used to examine differentially expressed proteins in oral squamous cell carcinoma (OSCC) tissues from Norway (n=15) and the UK (n=45). Twenty-nine proteins were found to be significantly overexpressed in the OSCCs examined compared to the normal controls. Identified proteins included, family of annexin proteins that play important roles in signal transduction pathways and regulation of cellular growth, keratin-1, heat-shock proteins (HSP), squamous cell carcinoma antigen (SCC-Ag), cytoskeleton proteins, and proteins involved in mitochondrial and intracellular signalling pathways.

Tumour-associated Proteins in Oral Squamous Cell Carcinomas by Proteomics.

Antibody microarrays, two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2-DE/MALDI-TOF-MS) were used to examine protein changes in 56 oral cancers (OCs)/normal controls (NCs) from Sudanese (41 OCs vs. 31 NCs) and Sri Lankan (15 OCs vs. 15 NCs) patients.

Recent Advances in Understanding Carcinogenicity of Oral Squamous Cell Carcinoma: From Basic Molecular Biology to Latest Genomic and Proteomic Findings.

The global increase in incidence and mortality, as well as the poor prognosis of oral squamous cell carcinoma (OSCC), has intensified efforts in the field of prevention and early detection of this disfiguring disease. Prevalence of OSCC is common in areas with high consumption of tobacco products and alcohol. Understanding the carcinogenicity of this cancer, using innovative techniques in genomic and proteomic analysis, is the main focus of current research in OSCC, and the hunt for potential molecular biomarkers is accelerating.

Mutations of the cell cycle regulatory genes p16INK4A and p21WAF1 and the metastasis-inducing gene S100A4 are infrequent and unrelated to p53 tumour suppressor gene status and data on survival in oropharyngeal squamous cell carcinomas.

Background: Oropharyngeal squamous cell carcinoma (SCC) is probably the most serious cancer to be encountered in the clinic.

Materials And Methods: Exons 1-3 of the p16 and exon 2 of the p21 genes were examined for mutations by polymerase chain reaction (PCR)/direct DNA sequencing methods in 21 fresh-frozen tissue specimens of oropharyngeal SCCs previously studied for expression and mutation (exons 5-9) of the p53 gene. Mutations in exons 2 and 3 of the S100A4 gene were studied in 10 cases.