[ Genotyping Characteristics of RhD-Negative Blood Donors in Wuhu Area].

Objective: To investigate the molecular mechanism and distribution characteristics of RhD negative phenotypes in Han population of blood donors in Wuhu city.

Methods: A total of 210 RhD samples from August 2021 to August 2022 were screened by serological test and collected from Wuhu Central Blood Station for the voluntary blood donor population. Exons 1 and 10 of the gene were amplificated by PCR to determine whether the samples had the gene.

A rapid identification method for common astigmatid species based on multiplex polymerase chain reaction.

Astigmatid mites are economically significant pests of stored products and sources of inhalant allergens causing allergic rhinitis and asthma worldwide. The morphological identification of astigmatid mites at the species level is often a difficult task due to their small size, phenotypic similarity and lack of diagnostic characters. We used multiplex polymerase chain reaction (PCR) to identify astigmatid mite species, which could complement the morphological data for the species-specific identification of mites.

Mitochondrial analysis of oribatid mites provides insights into their atypical tRNA annotation, genome rearrangement and evolution.

Background: The mitochondrial (mt) genomes of Sarcoptiformes mites typically contain 37 genes. Although the loss of genes is rare in Sarcoptiformes mite mitogenomes, two of the six previously reported oribatid mites (Acariforms: Sarcoptiformes) are reported to have lost parts of their tRNA genes. To confirm whether the tRNA genes were indeed lost and whether the loss is universal, we re-annotated the available oribatid mite sequences and sequenced the mitogenome of Oribatula sakamorii.

De novo sequence of the mitochondrial genome of Tyrophagus putrescentiae (Acari: Sarcoptiformes) including 22 tRNA sequences and the largest non-coding region.

In this study, we de novo sequenced and analyzed the circular mitochondrial genome (mitogenome) of Tyrophagus putrescentiae. It was 14,156 bp long and contained a complete set of 37 genes, contrary to the initial published sequences; it included 22 tRNA sequences and the largest non-coding region. The mtDNA gene order of T.

[Human skin infestation caused by : a case report].

Our study reports a case of acarodermatitis caused by . By morphological observations, the mites seized were identified as (deutonymph) which could attack humans resulting in acarodermatitis characterized with the symptoms of papules and blisters in different degrees. The patient was treated with 15% calamine lotion and anti-inflammatory and antipruritic drugs.

[Comparison of three different methods for isolating RNA from ].

Objective: To compare the effects of three kinds of RNA extraction methods, namely a modified SDS method, TRIzol reagent method, and CTAB method, so as to obtain an economical and efficient method for RNA extraction from .

Methods: The modified SDS method, TRIzol reagent method and CTAB method were applied to extract the RNA from . A nucleic acid protein analyzer was used to measure the concentration and purity of RNA.

[Study on natural population dynamics and spatial distribution pattern of in stored flour].

Objective: To understand the natural population dynamics and spatial distribution of in storage flour, so as to provide an evidence for its prevention and control.

Methods: The samples from five sampling points in Wuhu City were collected monthly from January to December, 2013, and examined and counted for . The dispersion pattern target, Iwao's m*- ̄; regression analysis and Taylor's lg-lg ̄; regression analysis were used for analyzing the spatial distribution pattern of in the storage flour.

[Optimization and comparison of extraction methods of mitochondrial DNA of ].

Objective: To optimize the extraction methods of mitochondrial genome DNA (mtDNA) of .

Methods: The pyrolysis, protein K variable-temperature digestion and high-concentration potassium acetate purification were applied to optimize the high-concentration-salt precipitation method, and then the optimized method was compared with two common extraction methods, the sucrose density gradient centrifugation method and traditional high-concentration-salt precipitation method. The mtDNA samples were identified by using spectrophotometry, agarose gel electrophoresis and the amplification products of COX1.

Association of Immune Factors with Drug-Resistant Tuberculosis: A Case-Control Study.

BACKGROUND Presently, studies of factors associated with drug-resistant tuberculosis (TB) focus on patients' socio-demographic characteristics and living habits, to the exclusion of biochemical indicators, especially immune factors. This study was carried out to determine whether immune factors are associated with drug-resistant TB. MATERIAL AND METHODS A total of 227 drug-resistant pulmonary TB patients and 225 drug-susceptible pulmonary TB patients were enrolled in this study.

[Population dynamics and spatial distribution pattern of ].

Objective: To characterize the population dynamics and spatial distribution of in the flour warehouse, so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitoring and management.

Methods: The samples from flour warehouses of four localities were collected, examined and counted for in every month in Wuhu City. The dispersion pattern target, Iwao / regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of in the flour warehouses.

[ and its hypopus found in feed of ].

Objective: To observe the and its hypopus in feed of .

Methods: Feed samples were collected from farms, and and its hypopus were separated from the samples by the vibration sieve method and floating method. The glass specimens were prepared and and its hypopus were observed under an optical microscope.

[Investigation of Acaroid mites breeding in stored dry fruits].

Objective: To study the species and density of Acaroid mites breeding in stored dry fruits.

Methods: The samples from the dried fruit stores and warehouses were collected, and the mites breeding in them were separated, then the slides with mites were prepared and observed by a light microscope for species identification and counting. The indexes such as the breeding density, species richness index, diversity index and evenness index were calculated.

[Investigation on mites and insects breeding status of bed mats in college dormitories in Wuhu City].

Objective: To understand the breeding situations of mites and insects from bed mats in dormitories in a college in Wuhu City, so as to provide evidences for improving the prevention and control of mites.

Methods: From March to May, 2015, the dust samples from bed mats in student dormitories were collected and detected for mites and insects by microscopy. In addition, the intervention measures including soaking the mats in the warm water, exposing the mats to the sunlight for a long time and cleaning up the indoor environment were carried out, and the breeding situations of mites and insects before and after the intervention were compared.

[Composition and diversity of Acaroid mites' communities in indoor environments of kindergartens in Wuhu City].

Objective: To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City, so as to provide the evidence for its prevention and control.

Methods: From March to June and September to December in 2014, dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated, identified and counted.

[Zoonotic trematode species identified in domestic animals from Huainan area].

Objective: To understand the zoonotic trematode species identified in domestic animals from Huainan area, Anhui Province, so as to supply the evidence for the prevention of zoonoses.

Methods: The livestock/definitive hosts were commercially available on category basis, and sacrificed for obtaining the parasitic samples via dissecting the viscera. The specimens were microscopically identified, with reference to the descriptions in the previous literature.

The complete mitochondrial genome of the brown leg mite, Aleuroglyphus ovatus (Acari: Sarcoptiformes): evaluation of largest non-coding region and unique tRNAs.

The circular mitochondrial genome (mitogenome) of Aleuroglyphus ovatus was sequenced. It was 14,328 bp long, and consisted of 37 coding genes including 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. This is the first description of the complete mitogenome of a species in the Acaridae (Acari: Sarcoptiformes).

[Investigation on species and community ecology of cheyletoid mites breeding in the stored traditional Chinese medicinal materials].

Objective: To investigate the species of Cheyletoid mites breeding status in the stored traditional Chinese medicinal materials and the relationship between its community and habitats.

Methods: A total of 60 samples of the traditional Chinese medicinal materials were collected from Huainan, Wuhu and Xuancheng in China. The mites were isolated with shakesieve shock, directicopy, Tullgren funnel, waternacopy and redricopy, and identified and counted under the light microscope.

Application of beta-(2-chloroaroyl) thioacetanilides in synthesis: an unusual and highly efficient access to thiochromeno[2,3-b]pyridine derivatives.

A series of unusual fused tricyclic thiochromeno[2,3-b]pyridines were successfully synthesized by tandem [3 + 3] annulation and SNAr of beta-(2-chloroaroyl) thioacetanilides with activated 4-arylidene-2-phenyloxazol-5(4H)-ones or aromatic aldehydes and ethyl 2-cyanoacetate under microwave irradiation, respectively. Because of the existence of the o-chloro atom of beta-(2-chloroaroyl) thioacetanilides, these reactions were very mild, convenient, and ortho-selective to form new fused tricyclic target molecules. In the domino processes, at least seven reactive distinct chemical sites were involved and up to three new covalent bonds and one tricycle with only cis configuration were generated.

[Survey on natural nidus of Metorchis orientalis in Huaihe River Basin].

Parafossarulus striatulus, Pseudorasbora parva and brood ducks are involved in the lifecycle of Metorchis orientalis. Natural nidi of M. orientalis are confirmed in Huaihe River Basin.