Non-invasive electrochemical immunosensor for reverse iontophoretic determination of cardiac troponins (cTnT & cTnI) in a simulated artificial skin model. Significance of raw DPV and CV data for chemometric discrimination.

Electrochemical immunosensors coupled with reverse iontophoresis (RI) for noninvasive determination of cardiac troponins were developed and validated according to ICH Q2 (R1) guideline. Linearity was in 0.01-10 and 100-500 ng/mL ranges.

Comparative Evaluation of Polyphenol Contents and Biological Activities of Five Cistus L. Species Native to Turkey.

In this study phytochemical compounds and antioxidant capacity, cytotoxic, antimicrobial and anti-biofilm activities of hydroethanolic extracts of five Cistus species (C. creticus L., C.

Oxidative Stress Biomarker Levels of Simulated Artificial Body Fluids: Comparative HPLC-PDA and LC-MS/MS Approaches: Significance of Raw PDA and MS/MS Data for Chemometric Discrimination.

HPLC-PDA, LC-MS/MS methods were developed for simultaneous determination of a group of oxidative stress biomarkers (OSBs); 2dA, 2dC, 2dU, 3NLT, 5HMU and 8OHdG in 10 simulated artificial body fluids. O-phosphoric acid and methanol composed mobile phases A and B for gradient elution in HPLC-PDA using ODS-2 column. Linearity obtained for 1.

Interactions between verapamil and digoxin in Langendorff-perfused rat hearts: the role of inhibition of P-glycoprotein in the heart.

P-glycoprotein (P-gp) is expressed in tumour cells as well as normal tissues including heart. Modulation of P-gp transport in vivo may lead to increased drug penetrance to tissues with resulting increases in toxicity. We aimed to investigate the effects of P-gp on the isolated heart by digoxin infusion in the absence and presence of verapamil.

Determination of the hydroxyl radical by its adduct formation with phenol and liquid chromatography/electrochemical detection.

An HPLC-ECD method is described for the indirect determination of the hydroxyl (OH) radical. Fenton's reaction is used to produce OH, which simultaneously attacks phenols (phenol or pyrocatechol) to form the adducts, pyrocatechol or pyrogallic acid. Thus, [OH] quantification is based on the separation and detection of pyrogallic acid and/or pyrocatechol by an isocratic HPLC-ECD method.

Flavonoids in Helichrysum pamphylicum inhibit mammalian type I DNA topoisomerase.

DNA topoisomerases are important targets for cancer chemotherapy. We investigated the effects of a methanolic extract of Helichrysum pamphylicum on mammalian DNA topoisomerase I via in vitro plasmid supercoil relaxation assays. The extracts manifested a considerable inhibition of the enzyme's activity in a dose-dependent manner.

Microelectrode for in vivo real-time detection of NO.

Nitric oxide (NO) is gaining importance with its diverse spectrum of clinic effects. However, there is still a need for an ideal sensor to monitor its concentration in tissue. An ideal sensor should not interfere with the ongoing physiological process, while making fast, reliable, and repeatable measurements.

Effect of melatonin cotreatment against kainic acid on coenzyme Q10, lipid peroxidation and Trx mRNA in rat hippocampus.

Oxidative stress is a likely molecular mechanism in the neurotoxicity of kainic acid (KA), an excitotoxic substance. The aim of this report was to assess the effect of melatonin co-treatment against KA by measuring the levels of Coenzyme Q10 (CoQ 10), lipid peroxidation (LPO), and Thioredoxin (Trx) mRNA in the rat hippocampus. The male rats were divided into three groups as saline, KA treatment (15 mg/kg), and KA plus melatonin (20 mg/kg).

Coenzyme Q10 concentrations in coronary artery disease.

Objectives: Ubiquinone-10 (CoQ10: the oxidized form of Coenyzme Q) is recognized as an antioxidant in the mitochondrial membrane and considered an anti-risk factor for coronary artery disease (CAD). The aim of this report is to determine the levels of plasma CoQ10 and the ratios of CoQ10 to plasma lipids in CAD patients and to compare them to a matched control group.

Methods: Sixty-four consecutive patients with CAD were studied and compared to 34 clinically healthy individuals.

Online electrochemical monitoring of nitric oxide during photodynamic therapy.

Photodynamic therapy (PDT), as a novel treatment modality, is based on the use of a photosensitizing agent with an excitation light source for the treatment of various malignancies. Its effect is mediated through reactive oxygen species and nitric oxide (NO), which are shown to be present in apoptosis. Individual differences among patients and even in different areas of the same tumor in one patient may cause a major problem with PDT: dose calculation during application of the light.

Comparison of electrochemical detection of acetylcholine-induced nitric oxide release (NO) and contractile force measurement of rabbit isolated carotid artery endothelium.

Since the identification of nitric oxide (NO) as an endothelial-derived relaxing factor, it became very important to quantify NO in biological models eventhough it is present in very low concentrations with a very short half-life. The use of electrochemistry as an alternative detection method is quite promising and electrochemical probes are now being developed to detect NO. This paper consists of an amperometric, bi-polymer modified, platinum-iridium microelectrode (Pt 90%-Ir 10% alloy, multistranded, total diameter 130 microm) design and its application for NO detection in acetylcholine (Ach) introduced, rabbit isolated carotid artery endothelium model.