Publications by authors named "Emmanuel Rosas-De-Paz"
Article Synopsis
- Systemic candidiasis is a severe opportunistic fungal infection primarily caused by specific yeast species, with increasing cases from non- species that may resist antifungal treatments.
- The study aimed to develop a PCR assay for quick detection of common pathogenic yeast species, focusing on blood and bronchial samples, using specially designed oligonucleotides that target a specific rDNA region.
- The newly developed PCR method demonstrated a sensitivity of 73.9%, a specificity of 96.3%, and strong predictive values, indicating its potential for reliable use in diagnosing systemic candidiasis.
The CSP (cell surface protein) microsatellite marker is useful for typing isolates and determining relationships at the subpopulation level because it has shown high discriminatory power. In the present study, 90 isolates from Mexico (MX), Argentina (AR), France (FR), and Peru (PE) were identified through a phylogenetic analysis using the gene fragment and were typed with the CSP microsatellite, and the types were identified using the nomenclature recommended in the literature. Genetic variability was analyzed through haplotype diversity, nucleotide diversity, polymorphic sites, and nucleotide differences between pairs of sequences.
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