Publications by authors named "Emma N Welbourne"
Following the recent COVID-19 pandemic, mRNA manufacturing processes are being actively developed and optimized to produce the next generation of mRNA vaccines and therapeutics. Herein, the performance of the tangential flow filtration (TFF) was evaluated for high-recovery, and high-purity separation of mRNA from unreacted nucleoside triphosphates (NTPs) from the in vitro transcription (IVT) reaction mixture. For the first time, the fouling model was successfully validated with TFF experimental data to describe the adsorption of mRNA on filtration membrane.
View Article and Find Full Text PDFQuality by design for mRNA platform purification based on continuous oligo-dT chromatography.
Mol Ther Nucleic Acids
December 2024
Article Synopsis
- * A new continuous oligo-dT chromatography process was developed, improving mRNA yield (over 90%), integrity (over 95%), and purity (over 99%), while also boosting productivity by 5.75 times and cutting costs by 15% compared to batch processing.
- * The optimization of the new process utilized a quality by design (QbD) framework to analyze relationships between important quality and performance factors, enhancing the overall chromatography process.
mRNA technology has recently demonstrated the ability to significantly change the timeline for developing and delivering a new vaccine from years to months. The potential of mRNA technology for rapid vaccine development has recently been highlighted by the successful development and approval of two mRNA vaccines for COVID-19. Importantly, this RNA-based approach holds promise for treatments beyond vaccines and infectious diseases, e.
View Article and Find Full Text PDFObjectives: To review urinary protein biomarkers as potential non-invasive, easily obtainable, early diagnostic tools in renal cell carcinoma (RCC).
Methods: A PubMed database search was performed up to the year 2020 to identify primary studies reporting potential urinary protein biomarkers for RCC. Separate searches were conducted to identify studies describing appropriate methods of developing cancer screening programmes and detection of cancer biomarkers.