Closed-perfusion transretinal ERG setup for preclinical drug and nanostructure testing.

Objective: The isolated mammalian retina may serve as a sensitive biosensor for preclinical drug testing, including eye drugs and a broader range of pharmaceuticals. To facilitate testing with minimal amounts of drug molecules or nanostructures, we developed a closed-perfusion transretinal electroretinography (tERG) setup.

Methods: The major challenge with small amounts of circulating perfusate was maintaining retinal viability and stability during long experiments.